Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models.
Genome manipulation in the mouse via microinjection of CRISPR/Cas9 site-specific nucleases has allowed the production time for genetically modified mouse models to be significantly reduced. Successful genome manipulation in the mouse has already been reported using Cas9 supplied by microinjection of...
Main Authors: | Alberto Cebrian-Serrano, Shijun Zha, Lars Hanssen, Daniel Biggs, Christopher Preece, Benjamin Davies |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC5231326?pdf=render |
Similar Items
-
Multiscale analysis of single and double maternal-zygotic Myh9 and Myh10 mutants during mouse preimplantation development
by: Markus Frederik Schliffka, et al.
Published: (2021-04-01) -
Mouse zygote-specific proteasome assembly chaperone important for maternal-to-zygotic transition
by: Seung-Wook Shin, et al.
Published: (2012-11-01) -
Efficient of Toll‐Like Receptor 4 Knockout in Mouse Zygotes by CRISPER/Cas9
by: Delsuz Rezaee, et al.
Published: (2021-08-01) -
On-Target CRISPR/Cas9 Activity Can Cause Undesigned Large Deletion in Mouse Zygotes
by: Alexey Korablev, et al.
Published: (2020-05-01) -
Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes
by: Takaya Abe, et al.
Published: (2020-05-01)