Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi
The aim of this study was to identify volatile and agar-diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus...
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doaj-6172f2d6a812441b8474469baf6a984c2020-11-24T23:59:32ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-10-0133548849810.5423/PPJ.OA.04.2017.0073PPJ.OA.04.2017.0073Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic FungiSeong Mi Lim0Mi-Young Yoon1Gyung Ja Choi2Yong Ho Choi3Kyoung Soo Jang4Teak Soo Shin5Hae Woong Park6Nan Hee Yu7Young Ho Kim8Jin-Cheol Kim9Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaEco-friendly New Material Research Group, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaEco-friendly New Material Research Group, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaEco-friendly New Material Research Group, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaEco-friendly New Material Research Group, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCrop Protection Research Center, Farm Hannong Company, Ltd., Chungnam 33010, KoreaR&D Division, World Institute of Kimchi, Gwangju 61755, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaDepartment of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, KoreaDivision of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, KoreaThe aim of this study was to identify volatile and agar-diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew, and red pepper anthracnose. Two antifungal compounds were isolated from strain G341 and identified as bacillomycin L and fengycin A by MS/MS analysis. Moreover, volatile compounds emitted from strain G341 were found to be able to inhibit mycelial growth of various phytopathogenic fungi. Based on volatile compound profiles of strain G341 obtained through headspace collection and analysis on GC-MS, dimethylsulfoxide, 1-butanol, and 3-hydroxy-2-butanone (acetoin) were identified. Taken together, these results suggest that B. valezensis G341 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624491antifungal metaboliteBacillus velezensisbiocontrolphytopathogenic fungi |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seong Mi Lim Mi-Young Yoon Gyung Ja Choi Yong Ho Choi Kyoung Soo Jang Teak Soo Shin Hae Woong Park Nan Hee Yu Young Ho Kim Jin-Cheol Kim |
spellingShingle |
Seong Mi Lim Mi-Young Yoon Gyung Ja Choi Yong Ho Choi Kyoung Soo Jang Teak Soo Shin Hae Woong Park Nan Hee Yu Young Ho Kim Jin-Cheol Kim Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi The Plant Pathology Journal antifungal metabolite Bacillus velezensis biocontrol phytopathogenic fungi |
author_facet |
Seong Mi Lim Mi-Young Yoon Gyung Ja Choi Yong Ho Choi Kyoung Soo Jang Teak Soo Shin Hae Woong Park Nan Hee Yu Young Ho Kim Jin-Cheol Kim |
author_sort |
Seong Mi Lim |
title |
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi |
title_short |
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi |
title_full |
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi |
title_fullStr |
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi |
title_full_unstemmed |
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi |
title_sort |
diffusible and volatile antifungal compounds produced by an antagonistic bacillus velezensis g341 against various phytopathogenic fungi |
publisher |
Hanrimwon Publishing Company |
series |
The Plant Pathology Journal |
issn |
1598-2254 |
publishDate |
2017-10-01 |
description |
The aim of this study was to identify volatile and agar-diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew, and red pepper anthracnose. Two antifungal compounds were isolated from strain G341 and identified as bacillomycin L and fengycin A by MS/MS analysis. Moreover, volatile compounds emitted from strain G341 were found to be able to inhibit mycelial growth of various phytopathogenic fungi. Based on volatile compound profiles of strain G341 obtained through headspace collection and analysis on GC-MS, dimethylsulfoxide, 1-butanol, and 3-hydroxy-2-butanone (acetoin) were identified. Taken together, these results suggest that B. valezensis G341 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi. |
topic |
antifungal metabolite Bacillus velezensis biocontrol phytopathogenic fungi |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5624491 |
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