Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda
In Uganda, bovine babesiosis continues to cause losses to the livestock industry because of shortages of cheap, quick, and reliable diagnostic tools to guide prescription measures. In this study, the presence of antibodies to <i>Babesia bigemina</i> and <i>Babesia bovis</i> i...
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doaj-617699fc0a2a4a1d8c2dc34e1a5fca8a2020-11-25T03:05:51ZengMDPI AGMicroorganisms2076-26072020-07-0181110111010.3390/microorganisms8081110Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in UgandaDickson Stuart Tayebwa0Amany Magdy Beshbishy1Gaber El-Saber Batiha2Mariam Komugisha3Byaruhanga Joseph4Patrick Vudriko5Ramadan Yahia6Luay Alkazmi7Helal F. Hetta8Naoaki Yokoyama9Ikuo Igarashi10National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 -13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 -13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 -13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanDepartment of Animal Health, Ministry of Agriculture, Animal Industry and Fisheries, Entebbe 513, UgandaRTC Laboratory, College of Veterinary Medicine, Animals’ Resources and Biosecurity, Makerere University, Kampala 7062, UgandaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 -13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanDepartment of Microbiology and Immunology, Faculty of pharmacy, Deraya University, Minia 11566, EgyptBiology Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, EgyptNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 -13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 -13, Inada-cho, Obihiro, Hokkaido 080-8555, JapanIn Uganda, bovine babesiosis continues to cause losses to the livestock industry because of shortages of cheap, quick, and reliable diagnostic tools to guide prescription measures. In this study, the presence of antibodies to <i>Babesia bigemina</i> and <i>Babesia bovis</i> in 401 bovine blood samples obtained from eastern and central areas of Uganda were detected using enzyme-linked immunosorbent assays (ELISAs) and immunochromatographic test strips (ICTs). The ELISA and ICT test used targeted the <i>B. bigemina</i> C-terminal rhoptry-associated protein (RAP-1/CT17) and <i>B. bovis</i> spherical body protein-4 (SPB-4). Using ELISA, single-ICT and dual-ICT, positive samples for <i>B. bovis</i> were detected in 25 (6.2%), 17 (4.3%), and 14 (3.7%) samples respectively, and positive samples for <i>B. bigemina</i> were detected in 34 (8.4%), 27 (6.7%), and 25 (6.2%), respectively. Additionally, a total of 13 animals (3.2%) had a mixed infection. The correlation between ELISA and single-ICT strips results revealed slight agreement with kappa values ranging from 0.088 to 0.191 between both methods, while the comparison between dual-ICT and single-ICT results showed very good agreement with kappa values >0.80. This study documented the seroprevalence of bovine babesiosis in central and eastern Uganda, and showed that ICT could, after further optimization, be a useful rapid diagnostic test for the diagnosis of bovine babesiosis in field settings.https://www.mdpi.com/2076-2607/8/8/1110bovine babesiosisELISAICT<i>B. bigemina</i> C-terminal rhoptry-associated protein (RAP-1/CT17)<i>B. bovis</i> spherical body protein-4 (SBP-4) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dickson Stuart Tayebwa Amany Magdy Beshbishy Gaber El-Saber Batiha Mariam Komugisha Byaruhanga Joseph Patrick Vudriko Ramadan Yahia Luay Alkazmi Helal F. Hetta Naoaki Yokoyama Ikuo Igarashi |
spellingShingle |
Dickson Stuart Tayebwa Amany Magdy Beshbishy Gaber El-Saber Batiha Mariam Komugisha Byaruhanga Joseph Patrick Vudriko Ramadan Yahia Luay Alkazmi Helal F. Hetta Naoaki Yokoyama Ikuo Igarashi Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda Microorganisms bovine babesiosis ELISA ICT <i>B. bigemina</i> C-terminal rhoptry-associated protein (RAP-1/CT17) <i>B. bovis</i> spherical body protein-4 (SBP-4) |
author_facet |
Dickson Stuart Tayebwa Amany Magdy Beshbishy Gaber El-Saber Batiha Mariam Komugisha Byaruhanga Joseph Patrick Vudriko Ramadan Yahia Luay Alkazmi Helal F. Hetta Naoaki Yokoyama Ikuo Igarashi |
author_sort |
Dickson Stuart Tayebwa |
title |
Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda |
title_short |
Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda |
title_full |
Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda |
title_fullStr |
Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda |
title_full_unstemmed |
Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda |
title_sort |
assessing the immunochromatographic test strip for serological detection of bovine babesiosis in uganda |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-07-01 |
description |
In Uganda, bovine babesiosis continues to cause losses to the livestock industry because of shortages of cheap, quick, and reliable diagnostic tools to guide prescription measures. In this study, the presence of antibodies to <i>Babesia bigemina</i> and <i>Babesia bovis</i> in 401 bovine blood samples obtained from eastern and central areas of Uganda were detected using enzyme-linked immunosorbent assays (ELISAs) and immunochromatographic test strips (ICTs). The ELISA and ICT test used targeted the <i>B. bigemina</i> C-terminal rhoptry-associated protein (RAP-1/CT17) and <i>B. bovis</i> spherical body protein-4 (SPB-4). Using ELISA, single-ICT and dual-ICT, positive samples for <i>B. bovis</i> were detected in 25 (6.2%), 17 (4.3%), and 14 (3.7%) samples respectively, and positive samples for <i>B. bigemina</i> were detected in 34 (8.4%), 27 (6.7%), and 25 (6.2%), respectively. Additionally, a total of 13 animals (3.2%) had a mixed infection. The correlation between ELISA and single-ICT strips results revealed slight agreement with kappa values ranging from 0.088 to 0.191 between both methods, while the comparison between dual-ICT and single-ICT results showed very good agreement with kappa values >0.80. This study documented the seroprevalence of bovine babesiosis in central and eastern Uganda, and showed that ICT could, after further optimization, be a useful rapid diagnostic test for the diagnosis of bovine babesiosis in field settings. |
topic |
bovine babesiosis ELISA ICT <i>B. bigemina</i> C-terminal rhoptry-associated protein (RAP-1/CT17) <i>B. bovis</i> spherical body protein-4 (SBP-4) |
url |
https://www.mdpi.com/2076-2607/8/8/1110 |
work_keys_str_mv |
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