Alternative transcript initiation and splicing as a response to DNA damage.
Humans are exposed to the DNA damaging agent, ionizing radiation (IR), from background radiation, medical treatments, occupational and accidental exposures. IR causes changes in transcription, but little is known about alternative transcription in response to IR on a genome-wide basis. These investi...
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2011-01-01
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doaj-618d75e3c9ec4d72836d6d2414cb24a02020-11-25T02:00:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2575810.1371/journal.pone.0025758Alternative transcript initiation and splicing as a response to DNA damage.Carl N SprungJason LiDaniel HovanMichael J McKayHelen B ForresterHumans are exposed to the DNA damaging agent, ionizing radiation (IR), from background radiation, medical treatments, occupational and accidental exposures. IR causes changes in transcription, but little is known about alternative transcription in response to IR on a genome-wide basis. These investigations examine the response to IR at the exon level in human cells, using exon arrays to comprehensively characterize radiation-induced transcriptional expression products. Previously uncharacterized alternative transcripts that preferentially occur following IR exposure have been discovered. A large number of genes showed alternative transcription initiation as a response to IR. Dose-response and time course kinetics have also been characterized. Interestingly, most genes showing alternative transcript induction maintained these isoforms over the dose range and times tested. Finally, clusters of co-ordinately up- and down-regulated radiation response genes were identified at specific chromosomal loci. These data provide the first genome-wide view of the transcriptional response to ionizing radiation at the exon level. This study provides novel insights into alternative transcripts as a mechanism for response to DNA damage and cell stress responses in general.http://europepmc.org/articles/PMC3198437?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carl N Sprung Jason Li Daniel Hovan Michael J McKay Helen B Forrester |
spellingShingle |
Carl N Sprung Jason Li Daniel Hovan Michael J McKay Helen B Forrester Alternative transcript initiation and splicing as a response to DNA damage. PLoS ONE |
author_facet |
Carl N Sprung Jason Li Daniel Hovan Michael J McKay Helen B Forrester |
author_sort |
Carl N Sprung |
title |
Alternative transcript initiation and splicing as a response to DNA damage. |
title_short |
Alternative transcript initiation and splicing as a response to DNA damage. |
title_full |
Alternative transcript initiation and splicing as a response to DNA damage. |
title_fullStr |
Alternative transcript initiation and splicing as a response to DNA damage. |
title_full_unstemmed |
Alternative transcript initiation and splicing as a response to DNA damage. |
title_sort |
alternative transcript initiation and splicing as a response to dna damage. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2011-01-01 |
description |
Humans are exposed to the DNA damaging agent, ionizing radiation (IR), from background radiation, medical treatments, occupational and accidental exposures. IR causes changes in transcription, but little is known about alternative transcription in response to IR on a genome-wide basis. These investigations examine the response to IR at the exon level in human cells, using exon arrays to comprehensively characterize radiation-induced transcriptional expression products. Previously uncharacterized alternative transcripts that preferentially occur following IR exposure have been discovered. A large number of genes showed alternative transcription initiation as a response to IR. Dose-response and time course kinetics have also been characterized. Interestingly, most genes showing alternative transcript induction maintained these isoforms over the dose range and times tested. Finally, clusters of co-ordinately up- and down-regulated radiation response genes were identified at specific chromosomal loci. These data provide the first genome-wide view of the transcriptional response to ionizing radiation at the exon level. This study provides novel insights into alternative transcripts as a mechanism for response to DNA damage and cell stress responses in general. |
url |
http://europepmc.org/articles/PMC3198437?pdf=render |
work_keys_str_mv |
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