Protection of Piglets with Maternally Derived Antibodies from Sows Inoculated with an Attenuated Live Marker Classical Swine Fever Vaccine (Flc-LOM-BE^rns)

• Main Authors: SeEun Choe, Jihye Shin, Ki-Sun Kim, Sok Song, Ra Mi Cha, Byung-Il Jung, Bang-Hun Hyun, Bong-Kyun Park, Dong-Jun An
• Format: Article
• Language: English
• Published: MDPI AG 2020-07-01
• Series: Pathogens
• Subjects: CSFV; DIVA; Flc-LOM-BE^rns vaccine; MDA
• Online Access: https://www.mdpi.com/2076-0817/9/8/608

Here, we investigated the protective efficacy provided by passive immunity induced by a classical swine fever (Flc-LOM-BE^rns) vaccine with the newly developed DIVA (Differentiating Infected from Vaccinated Animals) function. Ten pigs (aged 40–60 days) with maternally derived antibodies (MDAs) obtained from sows inoculated with the Flc-LOM-BE^rns vaccine were challenged with virulent classical swine fever virus (CSFV). Pigs with an MDA titer of 6 log₂ induced by the Flc-LOM-BE^rns vaccine were fully protected against virulent CSFV challenge but not the pigs with an MDA titer under 5 log₂. In addition, Flc-LOM-BE^rns vaccine-derived MDAs successfully differentiated vaccinated pigs by bovine viral diarrhea virus (BVDV) E^rns/CSFV E^rns antibody detection, functioning as a DIVA.