In vitro assessment of metabolic profile of Enterococcus strains of human origin

• Main Authors: Ashlesha Bhagwat, Uday S. Annapure
• Format: Article
• Language: English
• Published: SpringerOpen 2019-11-01
• Series: Journal of Genetic Engineering and Biotechnology
• Subjects: Enterococcus; Enzymes; BA; BSH; CLA; Antioxidant
• Online Access: http://link.springer.com/article/10.1186/s43141-019-0009-0

Abstract Background In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Results Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml−1) and sunflower oil (highest yield 48 μg ml−1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. Conclusion The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.