Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken
The chicken is a common type of poultry that is economically important both for its medicinal and nutritional values. Previous studies have found that free-range chickens have more skeletal muscle mass. The methyltransferase-like 21C gene (<i>METTL21C)</i> plays an important role in musc...
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doaj-61b7c99ed4ac4685a855573289ba8a932020-11-25T00:58:54ZengMDPI AGGenes2073-44252019-08-0110862810.3390/genes10080628genes10080628Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in ChickenGe Yang0Hongzhao Lu1Ling Wang2Jiarong Zhao3Wenxian Zeng4Tao Zhang5School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, ChinaSchool of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, ChinaThe chicken is a common type of poultry that is economically important both for its medicinal and nutritional values. Previous studies have found that free-range chickens have more skeletal muscle mass. The methyltransferase-like 21C gene (<i>METTL21C)</i> plays an important role in muscle development; however, there have been few reports on the role of <i>METTL21C</i> in chickens. In this study, we performed a genome-wide identification of chicken <i>METTL21C</i> genes and analyzed their phylogeny, transcriptional expression profile, and real-time quantitative polymerase chain reaction (qPCR). We identified 10 <i>GgMETTL21C</i> genes from chickens, 11 from mice, and 32 from humans, and these genes were divided into six groups, which showed a large amount of variation among these three species. A total of 15 motifs were detected in <i>METTL21C</i> genes, and the intron phase of the gene structure showed that the <i>METTL21C</i> gene family was conservative in evolution. Further, both the transcript data and qPCR showed that a single gene’s (<i>GgMETTL21C3)</i> expression level increased with the muscle development of chickens, indicating that the <i>METTL21C</i> genes are involved in the development of chicken muscles. Our results provide some reference value for the subsequent study of the function of <i>METTL21C.</i>https://www.mdpi.com/2073-4425/10/8/628chicken<i>METTL21C</i>evolutionexpression profiling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ge Yang Hongzhao Lu Ling Wang Jiarong Zhao Wenxian Zeng Tao Zhang |
spellingShingle |
Ge Yang Hongzhao Lu Ling Wang Jiarong Zhao Wenxian Zeng Tao Zhang Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken Genes chicken <i>METTL21C</i> evolution expression profiling |
author_facet |
Ge Yang Hongzhao Lu Ling Wang Jiarong Zhao Wenxian Zeng Tao Zhang |
author_sort |
Ge Yang |
title |
Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken |
title_short |
Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken |
title_full |
Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken |
title_fullStr |
Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken |
title_full_unstemmed |
Genome-Wide Identification and Transcriptional Expression of the <i>METTL21C</i> Gene Family in Chicken |
title_sort |
genome-wide identification and transcriptional expression of the <i>mettl21c</i> gene family in chicken |
publisher |
MDPI AG |
series |
Genes |
issn |
2073-4425 |
publishDate |
2019-08-01 |
description |
The chicken is a common type of poultry that is economically important both for its medicinal and nutritional values. Previous studies have found that free-range chickens have more skeletal muscle mass. The methyltransferase-like 21C gene (<i>METTL21C)</i> plays an important role in muscle development; however, there have been few reports on the role of <i>METTL21C</i> in chickens. In this study, we performed a genome-wide identification of chicken <i>METTL21C</i> genes and analyzed their phylogeny, transcriptional expression profile, and real-time quantitative polymerase chain reaction (qPCR). We identified 10 <i>GgMETTL21C</i> genes from chickens, 11 from mice, and 32 from humans, and these genes were divided into six groups, which showed a large amount of variation among these three species. A total of 15 motifs were detected in <i>METTL21C</i> genes, and the intron phase of the gene structure showed that the <i>METTL21C</i> gene family was conservative in evolution. Further, both the transcript data and qPCR showed that a single gene’s (<i>GgMETTL21C3)</i> expression level increased with the muscle development of chickens, indicating that the <i>METTL21C</i> genes are involved in the development of chicken muscles. Our results provide some reference value for the subsequent study of the function of <i>METTL21C.</i> |
topic |
chicken <i>METTL21C</i> evolution expression profiling |
url |
https://www.mdpi.com/2073-4425/10/8/628 |
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1725219942621511680 |