Identification and targeted disruption of the mouse gene encoding ESG1 (PH34/ECAT2/DPPA5)

• Main Authors: Ichisaka Tomoko, Maruyama Masayoshi, Itakura Ken, Amano Hisayuki, Nakagawa Masato, Yamanaka Shinya
• Format: Article
• Language: English
• Published: BMC 2006-02-01
• Series: BMC Developmental Biology
• Online Access: http://www.biomedcentral.com/1471-213X/6/11

<p>Abstract</p> <p>Background</p> <p>Embryonic stem cell-specific gene (<it>ESG</it>) 1, which encodes a KH-domain containing protein, is specifically expressed in early embryos, germ cells, and embryonic stem (ES) cells. Previous studies identified genomic clones containing the mouse <it>ESG1 </it>gene and five pseudogenes. However, their chromosomal localizations or physiological functions have not been determined.</p> <p>Results</p> <p>A Blast search of mouse genomic databases failed to locate the <it>ESG1 </it>gene. We identified several bacterial artificial clones containing the mouse <it>ESG1 </it>gene and an additional <it>ESG1</it>-like sequence with a similar gene structure from chromosome 9. The ESG1-like sequence contained a multiple critical mutations, indicating that it was a duplicated pseudogene. The 5' flanking region of the <it>ESG1 </it>gene, but not that of the pseudogene, exhibited strong enhancer and promoter activity in undifferentiated ES cells by luciferase reporter assay. To study the physiological functions of the <it>ESG1 </it>gene, we replaced this sequence in ES cells with a β-geo cassette by homologous recombination. Despite specific expression in early embryos and germ cells, <it>ESG1</it>^-/-mice developed normally and were fertile. We also generated <it>ESG1</it>^-/-ES cells both by a second independent homologous recombination and directly from blastocysts derived from heterozygous intercrosses. Northern blot and western blot analyses confirmed the absence of ESG1 in these cells. These ES cells demonstrated normal morphology, proliferation, and differentiation.</p> <p>Conclusion</p> <p>The mouse <it>ESG1 </it>gene, together with a duplicated pseudogene, is located on chromosome 9. Despite its specific expression in pluripotent cells and germ cells, ESG1 is dispensable for self-renewal of ES cells and establishment of germcells.</p>