Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy
Non-alcoholic fatty liver disease is a highly prevalent condition worldwide that increases the risk to develop liver fibrosis, cirrhosis, and hepatocellular carcinoma. Thus, it is imperative to develop novel diagnostic tools that together with liver biopsy help to differentiate mild and advanced deg...
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MDPI AG
2019-08-01
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Series: | Molecules |
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Online Access: | https://www.mdpi.com/1420-3049/24/17/3150 |
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doaj-61ce4bc822794c879fc05f0889f102f6 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alma Valor Eduardo J. Arista Romeu Galileo Escobedo Adriana Campos-Espinosa Ivette Irais Romero-Bello Javier Moreno-González Diego A. Fabila Bustos Suren Stolik Jose Manuel de la Rosa Vázquez Carolina Guzmán |
spellingShingle |
Alma Valor Eduardo J. Arista Romeu Galileo Escobedo Adriana Campos-Espinosa Ivette Irais Romero-Bello Javier Moreno-González Diego A. Fabila Bustos Suren Stolik Jose Manuel de la Rosa Vázquez Carolina Guzmán Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy Molecules endogenous fluorescence spectroscopy liver steatosis multi-variate analysis |
author_facet |
Alma Valor Eduardo J. Arista Romeu Galileo Escobedo Adriana Campos-Espinosa Ivette Irais Romero-Bello Javier Moreno-González Diego A. Fabila Bustos Suren Stolik Jose Manuel de la Rosa Vázquez Carolina Guzmán |
author_sort |
Alma Valor |
title |
Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy |
title_short |
Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy |
title_full |
Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy |
title_fullStr |
Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy |
title_full_unstemmed |
Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence Spectroscopy |
title_sort |
study of methionine choline deficient diet-induced steatosis in mice using endogenous fluorescence spectroscopy |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2019-08-01 |
description |
Non-alcoholic fatty liver disease is a highly prevalent condition worldwide that increases the risk to develop liver fibrosis, cirrhosis, and hepatocellular carcinoma. Thus, it is imperative to develop novel diagnostic tools that together with liver biopsy help to differentiate mild and advanced degrees of steatosis. Ex-vivo liver samples were collected from mice fed a methionine-choline deficient diet for two or eight weeks, and from a control group. The degree of hepatic steatosis was histologically evaluated, and fat content was assessed by Oil-Red O staining. On the other hand, fluorescence spectroscopy was used for the assessment of the steatosis progression. Fluorescence spectra were recorded at excitation wavelengths of 330, 365, 385, 405, and 415 nm by establishing surface contact of the fiber optic probe with the liver specimens. A multi-variate statistical approach based on principal component analysis followed by quadratic discriminant analysis was applied to spectral data to obtain classifiers able to distinguish mild and moderate stages of steatosis at the different excitation wavelengths. Receiver Operating Characteristic (ROC) curves were computed to compare classifier’s performances for each one of the five excitation wavelengths and steatosis stages. Optimal sensitivity and specificity were calculated from the corresponding ROC curves using the Youden index. Intensity in the endogenous fluorescence spectra at the given wavelengths progressively increased according to the time of exposure to diet. The area under the curve of the spectra was able to discriminate control liver samples from those with steatosis and differentiate among the time of exposure to the diet for most of the used excitation wavelengths. High specificities and sensitivities were obtained for every case; however, fluorescence spectra obtained by exciting with 405 nm yielded the best results distinguishing between the mentioned classes with a total classification error of 1.5% and optimal sensitivities and specificities better than 98.6% and 99.3%, respectively. |
topic |
endogenous fluorescence spectroscopy liver steatosis multi-variate analysis |
url |
https://www.mdpi.com/1420-3049/24/17/3150 |
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doaj-61ce4bc822794c879fc05f0889f102f62020-11-25T01:31:48ZengMDPI AGMolecules1420-30492019-08-012417315010.3390/molecules24173150molecules24173150Study of Methionine Choline Deficient Diet-Induced Steatosis in Mice Using Endogenous Fluorescence SpectroscopyAlma Valor0Eduardo J. Arista Romeu1Galileo Escobedo2Adriana Campos-Espinosa3Ivette Irais Romero-Bello4Javier Moreno-González5Diego A. Fabila Bustos6Suren Stolik7Jose Manuel de la Rosa Vázquez8Carolina Guzmán9Laboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico 07738, MexicoLaboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico 07738, MexicoLaboratorio de Proteómica, Dirección de Investigación, Hospital General de Mexico “Dr. Eduardo Liceaga”, Ciudad de Mexico 06720, MexicoLaboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Mexico/Hospital General de Mexico “Dr. Eduardo Liceaga”, Ciudad de Mexico 06720, MexicoLaboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Mexico/Hospital General de Mexico “Dr. Eduardo Liceaga”, Ciudad de Mexico 06720, MexicoLaboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Mexico/Hospital General de Mexico “Dr. Eduardo Liceaga”, Ciudad de Mexico 06720, MexicoLaboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico 07738, MexicoLaboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico 07738, MexicoLaboratorio de Biofotónica, ESIME Zac, Instituto Politécnico Nacional, Ciudad de Mexico 07738, MexicoLaboratorio de Hígado, Páncreas y Motilidad, Unidad de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de Mexico/Hospital General de Mexico “Dr. Eduardo Liceaga”, Ciudad de Mexico 06720, MexicoNon-alcoholic fatty liver disease is a highly prevalent condition worldwide that increases the risk to develop liver fibrosis, cirrhosis, and hepatocellular carcinoma. Thus, it is imperative to develop novel diagnostic tools that together with liver biopsy help to differentiate mild and advanced degrees of steatosis. Ex-vivo liver samples were collected from mice fed a methionine-choline deficient diet for two or eight weeks, and from a control group. The degree of hepatic steatosis was histologically evaluated, and fat content was assessed by Oil-Red O staining. On the other hand, fluorescence spectroscopy was used for the assessment of the steatosis progression. Fluorescence spectra were recorded at excitation wavelengths of 330, 365, 385, 405, and 415 nm by establishing surface contact of the fiber optic probe with the liver specimens. A multi-variate statistical approach based on principal component analysis followed by quadratic discriminant analysis was applied to spectral data to obtain classifiers able to distinguish mild and moderate stages of steatosis at the different excitation wavelengths. Receiver Operating Characteristic (ROC) curves were computed to compare classifier’s performances for each one of the five excitation wavelengths and steatosis stages. Optimal sensitivity and specificity were calculated from the corresponding ROC curves using the Youden index. Intensity in the endogenous fluorescence spectra at the given wavelengths progressively increased according to the time of exposure to diet. The area under the curve of the spectra was able to discriminate control liver samples from those with steatosis and differentiate among the time of exposure to the diet for most of the used excitation wavelengths. High specificities and sensitivities were obtained for every case; however, fluorescence spectra obtained by exciting with 405 nm yielded the best results distinguishing between the mentioned classes with a total classification error of 1.5% and optimal sensitivities and specificities better than 98.6% and 99.3%, respectively.https://www.mdpi.com/1420-3049/24/17/3150endogenous fluorescence spectroscopyliver steatosismulti-variate analysis |