Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model
Absorbable extracellular matrix (ECM) membrane has recently been used as a barrier membrane (BM) in guided tissue regeneration (GTR) and guided bone regeneration (GBR). Absorbable BMs are mostly based on collagen, which is more biocompatible than synthetic materials. However, implanted absorbable BM...
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doaj-61df213c1c32417cb6dff5bdae62469a2020-11-24T23:02:11ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/67152956715295Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect ModelJin Wook Hwang0Sungtae Kim1Se Won Kim2Jong Ho Lee3Department of New Materials, Oscotec Inc., Seongnam-si 13488, Republic of KoreaDepartment of Periodontology, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of KoreaDepartment of New Materials, Oscotec Inc., Seongnam-si 13488, Republic of KoreaDepartment of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of KoreaAbsorbable extracellular matrix (ECM) membrane has recently been used as a barrier membrane (BM) in guided tissue regeneration (GTR) and guided bone regeneration (GBR). Absorbable BMs are mostly based on collagen, which is more biocompatible than synthetic materials. However, implanted absorbable BMs can be rapidly degraded by enzymes in vivo. In a previous study, to delay degradation time, collagen fibers were treated with cross-linking agents. These compounds prevented the enzymatic degradation of BMs. However, cross-linked BMs can exhibit delayed tissue integration. In addition, the remaining cross-linker could induce inflammation. Here, we attempted to overcome these problems using a natural ECM membrane. The membrane consisted of freshly harvested porcine pericardium that was stripped from cells and immunoreagents by a cleaning process. Acellular porcine pericardium (APP) showed a bilayer structure with a smooth upper surface and a significantly coarser bottom layer. APP is an ECM with a thin layer (0.18–0.35 mm) but with excellent mechanical properties. Tensile strength of APP was 14.15±2.24 MPa. In in vivo experiments, APP was transplanted into rabbit tibia. The biocompatible material was retained for up to 3 months without the need for cross-linking. Therefore, we conclude that APP could support osteogenesis as a BM for up to 3 months.http://dx.doi.org/10.1155/2016/6715295 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Wook Hwang Sungtae Kim Se Won Kim Jong Ho Lee |
spellingShingle |
Jin Wook Hwang Sungtae Kim Se Won Kim Jong Ho Lee Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model BioMed Research International |
author_facet |
Jin Wook Hwang Sungtae Kim Se Won Kim Jong Ho Lee |
author_sort |
Jin Wook Hwang |
title |
Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model |
title_short |
Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model |
title_full |
Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model |
title_fullStr |
Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model |
title_full_unstemmed |
Effect of Extracellular Matrix Membrane on Bone Formation in a Rabbit Tibial Defect Model |
title_sort |
effect of extracellular matrix membrane on bone formation in a rabbit tibial defect model |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2016-01-01 |
description |
Absorbable extracellular matrix (ECM) membrane has recently been used as a barrier membrane (BM) in guided tissue regeneration (GTR) and guided bone regeneration (GBR). Absorbable BMs are mostly based on collagen, which is more biocompatible than synthetic materials. However, implanted absorbable BMs can be rapidly degraded by enzymes in vivo. In a previous study, to delay degradation time, collagen fibers were treated with cross-linking agents. These compounds prevented the enzymatic degradation of BMs. However, cross-linked BMs can exhibit delayed tissue integration. In addition, the remaining cross-linker could induce inflammation. Here, we attempted to overcome these problems using a natural ECM membrane. The membrane consisted of freshly harvested porcine pericardium that was stripped from cells and immunoreagents by a cleaning process. Acellular porcine pericardium (APP) showed a bilayer structure with a smooth upper surface and a significantly coarser bottom layer. APP is an ECM with a thin layer (0.18–0.35 mm) but with excellent mechanical properties. Tensile strength of APP was 14.15±2.24 MPa. In in vivo experiments, APP was transplanted into rabbit tibia. The biocompatible material was retained for up to 3 months without the need for cross-linking. Therefore, we conclude that APP could support osteogenesis as a BM for up to 3 months. |
url |
http://dx.doi.org/10.1155/2016/6715295 |
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