The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ di...
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2019-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-09809-3 |
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doaj-61e178a4b78e40d9866c7f7e1c00ad4a2021-05-11T12:10:22ZengNature Publishing GroupNature Communications2041-17232019-04-0110111310.1038/s41467-019-09809-3The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cellsWeihua Tian0Zilu Ye1Shengjun Wang2Morten Alder Schulz3Julie Van Coillie4Lingbo Sun5Yen-Hsi Chen6Yoshiki Narimatsu7Lars Hansen8Claus Kristensen9Ulla Mandel10Eric Paul Bennett11Siamak Jabbarzadeh-Tabrizi12Raphael Schiffmann13Jin-Song Shen14Sergey Y. Vakhrushev15Henrik Clausen16Zhang Yang17Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenGlycoDisplay ApSCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenInstitute of Metabolic Disease, Baylor Scott & White Research InstituteInstitute of Metabolic Disease, Baylor Scott & White Research InstituteInstitute of Metabolic Disease, Baylor Scott & White Research InstituteCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenCopenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of Health Sciences, University of CopenhagenLysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ distribution.https://doi.org/10.1038/s41467-019-09809-3 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Weihua Tian Zilu Ye Shengjun Wang Morten Alder Schulz Julie Van Coillie Lingbo Sun Yen-Hsi Chen Yoshiki Narimatsu Lars Hansen Claus Kristensen Ulla Mandel Eric Paul Bennett Siamak Jabbarzadeh-Tabrizi Raphael Schiffmann Jin-Song Shen Sergey Y. Vakhrushev Henrik Clausen Zhang Yang |
| spellingShingle |
Weihua Tian Zilu Ye Shengjun Wang Morten Alder Schulz Julie Van Coillie Lingbo Sun Yen-Hsi Chen Yoshiki Narimatsu Lars Hansen Claus Kristensen Ulla Mandel Eric Paul Bennett Siamak Jabbarzadeh-Tabrizi Raphael Schiffmann Jin-Song Shen Sergey Y. Vakhrushev Henrik Clausen Zhang Yang The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells Nature Communications |
| author_facet |
Weihua Tian Zilu Ye Shengjun Wang Morten Alder Schulz Julie Van Coillie Lingbo Sun Yen-Hsi Chen Yoshiki Narimatsu Lars Hansen Claus Kristensen Ulla Mandel Eric Paul Bennett Siamak Jabbarzadeh-Tabrizi Raphael Schiffmann Jin-Song Shen Sergey Y. Vakhrushev Henrik Clausen Zhang Yang |
| author_sort |
Weihua Tian |
| title |
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells |
| title_short |
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells |
| title_full |
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells |
| title_fullStr |
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells |
| title_full_unstemmed |
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells |
| title_sort |
glycosylation design space for recombinant lysosomal replacement enzymes produced in cho cells |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2019-04-01 |
| description |
Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ distribution. |
| url |
https://doi.org/10.1038/s41467-019-09809-3 |
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