Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii

The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated w...

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Main Authors:	Maria Sindhura John, Joseph Amruthraj Nagoth, Kesava Priyan Ramasamy, Alessio Mancini, Gabriele Giuli, Antonino Natalello, Patrizia Ballarini, Cristina Miceli, Sandra Pucciarelli
Format:	Article
Language:	English
Published:	MDPI AG 2020-01-01
Series:	Marine Drugs
Subjects:	green synthesis biomaterials silver nitrate antibiotics nanotechnology
Online Access:	https://www.mdpi.com/1660-3397/18/1/38

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spelling	doaj-61e2dea22730469eb80414ea266a524f2020-11-25T00:30:22ZengMDPI AGMarine Drugs1660-33972020-01-011813810.3390/md18010038md18010038Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>Maria Sindhura John0Joseph Amruthraj Nagoth1Kesava Priyan Ramasamy2Alessio Mancini3Gabriele Giuli4Antonino Natalello5Patrizia Ballarini6Cristina Miceli7Sandra Pucciarelli8School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalySchool of Sciences and Technology, University of Camerino, 62032 Camerino, ItalyDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milano, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalySchool of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, ItalyThe synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new <i>Pseudomonas</i> strain isolated from a consortium associated with the Antarctic marine ciliate <i>Euplotes focardii</i>. After incubation of <i>Pseudomonas</i> cultures with 1 mM of AgNO<sub>3</sub> at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20−70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and <i>Pseudomonas</i> AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including <i>Escherichia coli, Staphylococcus aureus</i> and <i>Candida albicans</i>. This study reports an efficient, rapid synthesis of stable AgNPs by a new <i>Pseudomonas</i> strain with high antimicrobial activity.https://www.mdpi.com/1660-3397/18/1/38green synthesis biomaterialssilver nitrateantibioticsnanotechnology
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli
spellingShingle	Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i> Marine Drugs green synthesis biomaterials silver nitrate antibiotics nanotechnology
author_facet	Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli
author_sort	Maria Sindhura John
title	Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>
title_short	Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>
title_full	Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>
title_fullStr	Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>
title_full_unstemmed	Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>
title_sort	synthesis of bioactive silver nanoparticles by a <i>pseudomonas</i> strain associated with the antarctic psychrophilic protozoon <i>euplotes focardii</i>
publisher	MDPI AG
series	Marine Drugs
issn	1660-3397
publishDate	2020-01-01
description	The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new <i>Pseudomonas</i> strain isolated from a consortium associated with the Antarctic marine ciliate <i>Euplotes focardii</i>. After incubation of <i>Pseudomonas</i> cultures with 1 mM of AgNO<sub>3</sub> at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20−70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and <i>Pseudomonas</i> AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including <i>Escherichia coli, Staphylococcus aureus</i> and <i>Candida albicans</i>. This study reports an efficient, rapid synthesis of stable AgNPs by a new <i>Pseudomonas</i> strain with high antimicrobial activity.
topic	green synthesis biomaterials silver nitrate antibiotics nanotechnology
url	https://www.mdpi.com/1660-3397/18/1/38
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Synthesis of Bioactive Silver Nanoparticles by a <i>Pseudomonas</i> Strain Associated with the Antarctic Psychrophilic Protozoon <i>Euplotes focardii</i>

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