Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)
Herein, we report a facile, economic, one-pot green synthesis of zinc oxide nanoparticles (ZnO-NPs) for diverse biomedical applications. In the study, ZnO-NPs were synthesized using an aqueous extract of Aquilegia pubiflora as an effective reducing and capping agent. The biomediated nanoparticles we...
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doaj-61f713743bac491895f276ed92ddfe822020-11-25T03:36:29ZengFrontiers Media S.A.Frontiers in Materials2296-80162020-08-01710.3389/fmats.2020.00249516743Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)Hasnain Jan0Muzamil Shah1Hazrat Usman2Muhammad Aslam Khan3Muhammad Aslam Khan4Muhammad Zia5Christophe Hano6Christophe Hano7Bilal Haider Abbasi8Bilal Haider Abbasi9Bilal Haider Abbasi10Department of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, International Islamic University, Islamabad, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, Université d’Orléans, CEDEX2, Orléans, FranceCOSMACTIFS, Bioactifs et Cosmétiques, CNRS GDR3711, Orléans, FranceDepartment of Biotechnology, Quaid-i-Azam University, Islamabad, PakistanLaboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, Université d’Orléans, CEDEX2, Orléans, FranceCOSMACTIFS, Bioactifs et Cosmétiques, CNRS GDR3711, Orléans, FranceHerein, we report a facile, economic, one-pot green synthesis of zinc oxide nanoparticles (ZnO-NPs) for diverse biomedical applications. In the study, ZnO-NPs were synthesized using an aqueous extract of Aquilegia pubiflora as an effective reducing and capping agent. The biomediated nanoparticles were characterized using various techniques including high-performance liquid chromatography (HPLC), X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, Dynamic light scattering, photoluminescence, and RAMAN. The particles were highly pure, having an average size of 34.23 nm with spherical or elliptical morphology, and displayed good aqueous dispersion capability. FTIR and HPLC confirmed the successful capping of flavonoids and hydroxycinnamic acid derivatives. The characterized NPs were then explored for their antimicrobial and anti-leishmanial potential. Among the tested bacterial and fungal strains, ZnO-NPs were more potent toward Pseudomonas aeruginosa and Fusarium solani with inhibition zone of 10.3 ± 0.19 mm and 13 ± 1.4 mm, respectively. A dose-dependent cytotoxic effect was observed against Leishmania tropica (KWH23) with significant IC50 for both the promastigote (48 μg/mL) and amastigote form (51 μg/mL) of the parasite. In addition, bacterial kinase enzymes were inhibited by ZnO-NPs, thus allowing us to elaborate a possible action mechanism. Finally, the remarkable biocompatible nature of the particles was confirmed against freshly isolated human red blood cells (hRBCs). Altogether, these results affirmed the high antimicrobial and antiparasitic potential of ZnO-NPs obtained through a biogenic synthetic approach using aqueous extract of the Himalayan Columbine (Aquilegia pubiflora).https://www.frontiersin.org/article/10.3389/fmats.2020.00249/fullzinc oxide nanoparticlesgreen synthesisnanomedicinebio-compatiblephytochemicalsantibacterial and antifungal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hasnain Jan Muzamil Shah Hazrat Usman Muhammad Aslam Khan Muhammad Aslam Khan Muhammad Zia Christophe Hano Christophe Hano Bilal Haider Abbasi Bilal Haider Abbasi Bilal Haider Abbasi |
spellingShingle |
Hasnain Jan Muzamil Shah Hazrat Usman Muhammad Aslam Khan Muhammad Aslam Khan Muhammad Zia Christophe Hano Christophe Hano Bilal Haider Abbasi Bilal Haider Abbasi Bilal Haider Abbasi Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora) Frontiers in Materials zinc oxide nanoparticles green synthesis nanomedicine bio-compatible phytochemicals antibacterial and antifungal |
author_facet |
Hasnain Jan Muzamil Shah Hazrat Usman Muhammad Aslam Khan Muhammad Aslam Khan Muhammad Zia Christophe Hano Christophe Hano Bilal Haider Abbasi Bilal Haider Abbasi Bilal Haider Abbasi |
author_sort |
Hasnain Jan |
title |
Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora) |
title_short |
Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora) |
title_full |
Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora) |
title_fullStr |
Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora) |
title_full_unstemmed |
Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora) |
title_sort |
biogenic synthesis and characterization of antimicrobial and antiparasitic zinc oxide (zno) nanoparticles using aqueous extracts of the himalayan columbine (aquilegia pubiflora) |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Materials |
issn |
2296-8016 |
publishDate |
2020-08-01 |
description |
Herein, we report a facile, economic, one-pot green synthesis of zinc oxide nanoparticles (ZnO-NPs) for diverse biomedical applications. In the study, ZnO-NPs were synthesized using an aqueous extract of Aquilegia pubiflora as an effective reducing and capping agent. The biomediated nanoparticles were characterized using various techniques including high-performance liquid chromatography (HPLC), X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, Dynamic light scattering, photoluminescence, and RAMAN. The particles were highly pure, having an average size of 34.23 nm with spherical or elliptical morphology, and displayed good aqueous dispersion capability. FTIR and HPLC confirmed the successful capping of flavonoids and hydroxycinnamic acid derivatives. The characterized NPs were then explored for their antimicrobial and anti-leishmanial potential. Among the tested bacterial and fungal strains, ZnO-NPs were more potent toward Pseudomonas aeruginosa and Fusarium solani with inhibition zone of 10.3 ± 0.19 mm and 13 ± 1.4 mm, respectively. A dose-dependent cytotoxic effect was observed against Leishmania tropica (KWH23) with significant IC50 for both the promastigote (48 μg/mL) and amastigote form (51 μg/mL) of the parasite. In addition, bacterial kinase enzymes were inhibited by ZnO-NPs, thus allowing us to elaborate a possible action mechanism. Finally, the remarkable biocompatible nature of the particles was confirmed against freshly isolated human red blood cells (hRBCs). Altogether, these results affirmed the high antimicrobial and antiparasitic potential of ZnO-NPs obtained through a biogenic synthetic approach using aqueous extract of the Himalayan Columbine (Aquilegia pubiflora). |
topic |
zinc oxide nanoparticles green synthesis nanomedicine bio-compatible phytochemicals antibacterial and antifungal |
url |
https://www.frontiersin.org/article/10.3389/fmats.2020.00249/full |
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