The Effect of Herbal Medicine on Colon Cancer Cells in Culture
Medicinal herbs are being increasingly recognized as useful complementary treatments for cancer. Colon cancer is the third most common cancer in men and the second in women worldwide. Colon cancer diseases is very heterogeneous in terms of grade, genetics, ploidy, and oncogene/tumor suppress or gene...
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doaj-6214fcf3cc29431982c2fb4b2ab447292020-11-24T21:47:07ZengMDPI AGProceedings2504-39002017-11-01110103310.3390/proceedings1101033proceedings1101033The Effect of Herbal Medicine on Colon Cancer Cells in CulturePelin Toros0Büşra Şen1Pınar K. Sönmez2Mahmud Özkut3Şamil Öztürk4Fatih Çöllü5Sevinç İnan6İbrahim Tuğlu7Department of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, TurkeyDepartment of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, TurkeyDepartment of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, TurkeyDepartment of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, TurkeyDepartment of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, TurkeyBiology, Faculty of Science and Literature, Manisa Celal Bayar University, 45140 Manisa, TurkeyDepartment of Histology & Embryology, Faculty of Medicine, İzmir University of Economics, 35330 İzmir, TurkeyDepartment of Histology & Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, TurkeyMedicinal herbs are being increasingly recognized as useful complementary treatments for cancer. Colon cancer is the third most common cancer in men and the second in women worldwide. Colon cancer diseases is very heterogeneous in terms of grade, genetics, ploidy, and oncogene/tumor suppress or gene expression and its biological, hormonal, and molecular characteristics are extremely complex. In this study our aim was to identify the effect of different medical plants Viscum album, Inula viscosa, Hypericum perforatum, Lysimachia nummularia, Oleocanthal, Pinus pinaster and Rubus caeisus on colon cancer cell line. Colo320 cancer cells and human adipose tissue derived mesenchymal stem cell were analysed for four medical plants in culture. Firstly, the cytotoxicity rate (IC50) determined by MTT. After that, immunocytochemical staining were done eNOS, VEGF and TUNEL method for apoptosis. The stainings were evaluated by H-score. As a result, Inula viscosa and Rubus caeisus have a higher inhibitory effect on cell proliferation and apoptosis in both cancers than the other medicinal plants. Colo320 cancer cells expressed strong eNOS by these plants and also both plants were not toxic for adipose tissue derived mesenchymal stem cells.https://www.mdpi.com/2504-3900/1/10/1033Colo 320Herbal MedicineTunelCancer Treatment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pelin Toros Büşra Şen Pınar K. Sönmez Mahmud Özkut Şamil Öztürk Fatih Çöllü Sevinç İnan İbrahim Tuğlu |
spellingShingle |
Pelin Toros Büşra Şen Pınar K. Sönmez Mahmud Özkut Şamil Öztürk Fatih Çöllü Sevinç İnan İbrahim Tuğlu The Effect of Herbal Medicine on Colon Cancer Cells in Culture Proceedings Colo 320 Herbal Medicine Tunel Cancer Treatment |
author_facet |
Pelin Toros Büşra Şen Pınar K. Sönmez Mahmud Özkut Şamil Öztürk Fatih Çöllü Sevinç İnan İbrahim Tuğlu |
author_sort |
Pelin Toros |
title |
The Effect of Herbal Medicine on Colon Cancer Cells in Culture |
title_short |
The Effect of Herbal Medicine on Colon Cancer Cells in Culture |
title_full |
The Effect of Herbal Medicine on Colon Cancer Cells in Culture |
title_fullStr |
The Effect of Herbal Medicine on Colon Cancer Cells in Culture |
title_full_unstemmed |
The Effect of Herbal Medicine on Colon Cancer Cells in Culture |
title_sort |
effect of herbal medicine on colon cancer cells in culture |
publisher |
MDPI AG |
series |
Proceedings |
issn |
2504-3900 |
publishDate |
2017-11-01 |
description |
Medicinal herbs are being increasingly recognized as useful complementary treatments for cancer. Colon cancer is the third most common cancer in men and the second in women worldwide. Colon cancer diseases is very heterogeneous in terms of grade, genetics, ploidy, and oncogene/tumor suppress or gene expression and its biological, hormonal, and molecular characteristics are extremely complex. In this study our aim was to identify the effect of different medical plants Viscum album, Inula viscosa, Hypericum perforatum, Lysimachia nummularia, Oleocanthal, Pinus pinaster and Rubus caeisus on colon cancer cell line. Colo320 cancer cells and human adipose tissue derived mesenchymal stem cell were analysed for four medical plants in culture. Firstly, the cytotoxicity rate (IC50) determined by MTT. After that, immunocytochemical staining were done eNOS, VEGF and TUNEL method for apoptosis. The stainings were evaluated by H-score. As a result, Inula viscosa and Rubus caeisus have a higher inhibitory effect on cell proliferation and apoptosis in both cancers than the other medicinal plants. Colo320 cancer cells expressed strong eNOS by these plants and also both plants were not toxic for adipose tissue derived mesenchymal stem cells. |
topic |
Colo 320 Herbal Medicine Tunel Cancer Treatment |
url |
https://www.mdpi.com/2504-3900/1/10/1033 |
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