Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids
Cellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods u...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2015-06-01
|
Series: | Engineering |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2095809916300728 |
id |
doaj-621f504c219243f789ae33188908fada |
---|---|
record_format |
Article |
spelling |
doaj-621f504c219243f789ae33188908fada2020-11-24T23:21:14ZengElsevierEngineering2095-80992015-06-011226927410.15302/J-ENG-2015062Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular SpheroidsKai Ling0Guoyou Huang1Juncong Liu2Xiaohui Zhang3Yufei Ma4Tianjian Lu5Feng Xu6State Key Laboratory for Strength and Vibration of Mechanical Structures, School of Aerospace, Xi'an Jiaotong University, Xi'an 710049, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, ChinaBioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an 710049, ChinaCellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cell-embedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening.http://www.sciencedirect.com/science/article/pii/S2095809916300728MCF-7 cellular spheroidsbioprintinghydrogelsconcave wellstissue on a chip |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kai Ling Guoyou Huang Juncong Liu Xiaohui Zhang Yufei Ma Tianjian Lu Feng Xu |
spellingShingle |
Kai Ling Guoyou Huang Juncong Liu Xiaohui Zhang Yufei Ma Tianjian Lu Feng Xu Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids Engineering MCF-7 cellular spheroids bioprinting hydrogels concave wells tissue on a chip |
author_facet |
Kai Ling Guoyou Huang Juncong Liu Xiaohui Zhang Yufei Ma Tianjian Lu Feng Xu |
author_sort |
Kai Ling |
title |
Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids |
title_short |
Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids |
title_full |
Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids |
title_fullStr |
Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids |
title_full_unstemmed |
Bioprinting-Based High-Throughput Fabrication of Three-Dimensional MCF-7 Human Breast Cancer Cellular Spheroids |
title_sort |
bioprinting-based high-throughput fabrication of three-dimensional mcf-7 human breast cancer cellular spheroids |
publisher |
Elsevier |
series |
Engineering |
issn |
2095-8099 |
publishDate |
2015-06-01 |
description |
Cellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted increasing interest for pathological study and drug-screening applications. Various methods, including microwells in particular, have been developed for engineering cellular spheroids. However, these methods usually suffer from either destructive molding operations or cell loss and non-uniform cell distribution among the wells due to two-step molding and cell seeding. We have developed a facile method that utilizes cell-embedded hydrogel arrays as templates for concave well fabrication and in situ MCF-7 cellular spheroid formation on a chip. A custom-built bioprinting system was applied for the fabrication of sacrificial gelatin arrays and sequentially concave wells in a high-throughput, flexible, and controlled manner. The ability to achieve in situ cell seeding for cellular spheroid construction was demonstrated with the advantage of uniform cell seeding and the potential for programmed fabrication of tissue models on chips. The developed method holds great potential for applications in tissue engineering, regenerative medicine, and drug screening. |
topic |
MCF-7 cellular spheroids bioprinting hydrogels concave wells tissue on a chip |
url |
http://www.sciencedirect.com/science/article/pii/S2095809916300728 |
work_keys_str_mv |
AT kailing bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids AT guoyouhuang bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids AT juncongliu bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids AT xiaohuizhang bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids AT yufeima bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids AT tianjianlu bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids AT fengxu bioprintingbasedhighthroughputfabricationofthreedimensionalmcf7humanbreastcancercellularspheroids |
_version_ |
1725572043633590272 |