Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound
Abstract Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired t...
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doaj-622039e1037d42bea95551d67f5f09b52020-12-08T03:26:36ZengNature Publishing GroupScientific Reports2045-23222018-05-018111410.1038/s41598-018-25937-0Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compoundJ.-W. Lackmann0K. Wende1C. Verlackt2J. Golda3J. Volzke4F. Kogelheide5J. Held6S. Bekeschus7A. Bogaerts8V. Schulz-von der Gathen9K. Stapelmann10Biomedical Applications of Plasma Technology, Ruhr University BochumZIK plasmatis, Leibniz-Institute for Plasma Science and TechnologyPLASMANT, University of AntwerpExperimental Physics II, Ruhr University BochumZIK plasmatis, Leibniz-Institute for Plasma Science and TechnologyBiomedical Applications of Plasma Technology, Ruhr University BochumExperimental Physics II, Ruhr University BochumZIK plasmatis, Leibniz-Institute for Plasma Science and TechnologyPLASMANT, University of AntwerpExperimental Physics II, Ruhr University BochumBiomedical Applications of Plasma Technology, Ruhr University BochumAbstract Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer on the molecular mechanisms underlying the observed effects and to enable the discrimination between different plasma sources. Here, an improved model to trace short-lived reactive species is presented. Using FTIR, high-resolution mass spectrometry, and molecular dynamics computational simulation, covalent modifications of cysteine treated with different plasmas were deciphered and the respective product pattern used to generate a fingerprint of each plasma source. Such, our experimental model allows a fast and reliable grading of the chemical potential of plasmas used for medical purposes. Major reaction products were identified to be cysteine sulfonic acid, cystine, and cysteine fragments. Less-abundant products, such as oxidized cystine derivatives or S-nitrosylated cysteines, were unique to different plasma sources or operating conditions. The data collected point at hydroxyl radicals, atomic O, and singlet oxygen as major contributing species that enable an impact on cellular thiol groups when applying cold plasma in vitro or in vivo.https://doi.org/10.1038/s41598-018-25937-0 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
J.-W. Lackmann K. Wende C. Verlackt J. Golda J. Volzke F. Kogelheide J. Held S. Bekeschus A. Bogaerts V. Schulz-von der Gathen K. Stapelmann |
spellingShingle |
J.-W. Lackmann K. Wende C. Verlackt J. Golda J. Volzke F. Kogelheide J. Held S. Bekeschus A. Bogaerts V. Schulz-von der Gathen K. Stapelmann Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound Scientific Reports |
author_facet |
J.-W. Lackmann K. Wende C. Verlackt J. Golda J. Volzke F. Kogelheide J. Held S. Bekeschus A. Bogaerts V. Schulz-von der Gathen K. Stapelmann |
author_sort |
J.-W. Lackmann |
title |
Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound |
title_short |
Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound |
title_full |
Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound |
title_fullStr |
Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound |
title_full_unstemmed |
Chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound |
title_sort |
chemical fingerprints of cold physical plasmas – an experimental and computational study using cysteine as tracer compound |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2018-05-01 |
description |
Abstract Reactive oxygen and nitrogen species released by cold physical plasma are being proposed as effectors in various clinical conditions connected to inflammatory processes. As these plasmas can be tailored in a wide range, models to compare and control their biochemical footprint are desired to infer on the molecular mechanisms underlying the observed effects and to enable the discrimination between different plasma sources. Here, an improved model to trace short-lived reactive species is presented. Using FTIR, high-resolution mass spectrometry, and molecular dynamics computational simulation, covalent modifications of cysteine treated with different plasmas were deciphered and the respective product pattern used to generate a fingerprint of each plasma source. Such, our experimental model allows a fast and reliable grading of the chemical potential of plasmas used for medical purposes. Major reaction products were identified to be cysteine sulfonic acid, cystine, and cysteine fragments. Less-abundant products, such as oxidized cystine derivatives or S-nitrosylated cysteines, were unique to different plasma sources or operating conditions. The data collected point at hydroxyl radicals, atomic O, and singlet oxygen as major contributing species that enable an impact on cellular thiol groups when applying cold plasma in vitro or in vivo. |
url |
https://doi.org/10.1038/s41598-018-25937-0 |
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