Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation.
Intracellular Toll-like receptors (TLRs) expressed by dendritic cells recognize nucleic acids derived from pathogens and play an important role in the immune responses against the influenza virus (IAV), a single-stranded RNA sensed by different receptors including TLR7. However, the importance of TL...
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2012-01-01
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doaj-623955a6ac4443deb2789d13f01914152021-04-21T17:27:12ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742012-01-0188e100284110.1371/journal.ppat.1002841Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation.Sophia MaschalidiSigne HässlerFany BlancFernando E SepulvedaMira TohmeMichel ChignardPeter van EndertMustapha Si-TaharDelphyne DescampsBénédicte ManouryIntracellular Toll-like receptors (TLRs) expressed by dendritic cells recognize nucleic acids derived from pathogens and play an important role in the immune responses against the influenza virus (IAV), a single-stranded RNA sensed by different receptors including TLR7. However, the importance of TLR7 processing in the development of anti-viral immune responses is not known. Here we report that asparagine endopeptidase (AEP) deficient mice are unable to generate a strong anti-IAV response, as demonstrated by reduced inflammation, cross presentation of cell-associated antigens and priming of CD8(+) T cells following TLR7-dependent pulmonary infection induced by IAV. Moreover, AEP deficient lung epithelial- or myeloid-cells exhibit impaired TLR7 signaling due to defective processing of this receptor. Indeed, TLR7 requires a proteolytic cleavage by AEP to generate a C-terminal fragment competent for signaling. Thus, AEP activity is critical for TLR7 processing, opening new possibilities for the treatment of influenza and TLR7-dependent inflammatory diseases.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22916010/pdf/?tool=EBI |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sophia Maschalidi Signe Hässler Fany Blanc Fernando E Sepulveda Mira Tohme Michel Chignard Peter van Endert Mustapha Si-Tahar Delphyne Descamps Bénédicte Manoury |
spellingShingle |
Sophia Maschalidi Signe Hässler Fany Blanc Fernando E Sepulveda Mira Tohme Michel Chignard Peter van Endert Mustapha Si-Tahar Delphyne Descamps Bénédicte Manoury Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation. PLoS Pathogens |
author_facet |
Sophia Maschalidi Signe Hässler Fany Blanc Fernando E Sepulveda Mira Tohme Michel Chignard Peter van Endert Mustapha Si-Tahar Delphyne Descamps Bénédicte Manoury |
author_sort |
Sophia Maschalidi |
title |
Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation. |
title_short |
Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation. |
title_full |
Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation. |
title_fullStr |
Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation. |
title_full_unstemmed |
Asparagine endopeptidase controls anti-influenza virus immune responses through TLR7 activation. |
title_sort |
asparagine endopeptidase controls anti-influenza virus immune responses through tlr7 activation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2012-01-01 |
description |
Intracellular Toll-like receptors (TLRs) expressed by dendritic cells recognize nucleic acids derived from pathogens and play an important role in the immune responses against the influenza virus (IAV), a single-stranded RNA sensed by different receptors including TLR7. However, the importance of TLR7 processing in the development of anti-viral immune responses is not known. Here we report that asparagine endopeptidase (AEP) deficient mice are unable to generate a strong anti-IAV response, as demonstrated by reduced inflammation, cross presentation of cell-associated antigens and priming of CD8(+) T cells following TLR7-dependent pulmonary infection induced by IAV. Moreover, AEP deficient lung epithelial- or myeloid-cells exhibit impaired TLR7 signaling due to defective processing of this receptor. Indeed, TLR7 requires a proteolytic cleavage by AEP to generate a C-terminal fragment competent for signaling. Thus, AEP activity is critical for TLR7 processing, opening new possibilities for the treatment of influenza and TLR7-dependent inflammatory diseases. |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22916010/pdf/?tool=EBI |
work_keys_str_mv |
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