Variation of antibiotic resistome during commercial livestock manure composting
Composting has been widely used to turn livestock manure into organic fertilizer. However, livestock manure contains various contaminants including antibiotics and antibiotic resistance genes (ARGs). Here we investigated the variation of antibiotic resistome and its influencing factors during a comm...
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Format: | Article |
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Elsevier
2020-03-01
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Series: | Environment International |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412019333057 |
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doaj-623aa7db87124027a5e58f804f5b7e49 |
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Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Min Zhang Liang-Ying He You-Sheng Liu Jian-Liang Zhao Jin-Na Zhang Jun Chen Qian-Qian Zhang Guang-Guo Ying |
spellingShingle |
Min Zhang Liang-Ying He You-Sheng Liu Jian-Liang Zhao Jin-Na Zhang Jun Chen Qian-Qian Zhang Guang-Guo Ying Variation of antibiotic resistome during commercial livestock manure composting Environment International |
author_facet |
Min Zhang Liang-Ying He You-Sheng Liu Jian-Liang Zhao Jin-Na Zhang Jun Chen Qian-Qian Zhang Guang-Guo Ying |
author_sort |
Min Zhang |
title |
Variation of antibiotic resistome during commercial livestock manure composting |
title_short |
Variation of antibiotic resistome during commercial livestock manure composting |
title_full |
Variation of antibiotic resistome during commercial livestock manure composting |
title_fullStr |
Variation of antibiotic resistome during commercial livestock manure composting |
title_full_unstemmed |
Variation of antibiotic resistome during commercial livestock manure composting |
title_sort |
variation of antibiotic resistome during commercial livestock manure composting |
publisher |
Elsevier |
series |
Environment International |
issn |
0160-4120 |
publishDate |
2020-03-01 |
description |
Composting has been widely used to turn livestock manure into organic fertilizer. However, livestock manure contains various contaminants including antibiotics and antibiotic resistance genes (ARGs). Here we investigated the variation of antibiotic resistome and its influencing factors during a commercial livestock manure composting. The results showed that composting could effectively reduce the relative abundance of ARGs and mobile genic elements (MGEs). As the dominant phylum in the composting samples, the key potential bacterial host of ARGs were Actinobacteria such as Leucobacter, Mycobacterium and Thermomonosporaceae unclassified. Meanwhile, Legionella pneumophila, Staphylococcus saprophyticus, Haemophilus ducreyi and Siccibacter turicensis may be the key potential pathogenic host of ARGs because of their co-occurrence with ARG subtypes. Redundancy analysis showed that the dissipation of ARGs during composting was linked to various environmental factors such as moisture. Bacterial succession as well as profile of biocide and metal resistance genes (BMRGs) were the determinants which constructed the antibiotic resistome during manure composting. However, the residues of ARGs and pathogens in compost products may still pose risks to human and crops after fertilization. Keywords: Antibiotic resistance genes, Livestock manure, Composting, Microbial community, Pathogens, Antibiotic resistome |
url |
http://www.sciencedirect.com/science/article/pii/S0160412019333057 |
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doaj-623aa7db87124027a5e58f804f5b7e492020-11-24T23:49:22ZengElsevierEnvironment International0160-41202020-03-01136Variation of antibiotic resistome during commercial livestock manure compostingMin Zhang0Liang-Ying He1You-Sheng Liu2Jian-Liang Zhao3Jin-Na Zhang4Jun Chen5Qian-Qian Zhang6Guang-Guo Ying7SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaState Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, University Town, Guangzhou 510006, China; Corresponding author at: SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou 510006, China.Composting has been widely used to turn livestock manure into organic fertilizer. However, livestock manure contains various contaminants including antibiotics and antibiotic resistance genes (ARGs). Here we investigated the variation of antibiotic resistome and its influencing factors during a commercial livestock manure composting. The results showed that composting could effectively reduce the relative abundance of ARGs and mobile genic elements (MGEs). As the dominant phylum in the composting samples, the key potential bacterial host of ARGs were Actinobacteria such as Leucobacter, Mycobacterium and Thermomonosporaceae unclassified. Meanwhile, Legionella pneumophila, Staphylococcus saprophyticus, Haemophilus ducreyi and Siccibacter turicensis may be the key potential pathogenic host of ARGs because of their co-occurrence with ARG subtypes. Redundancy analysis showed that the dissipation of ARGs during composting was linked to various environmental factors such as moisture. Bacterial succession as well as profile of biocide and metal resistance genes (BMRGs) were the determinants which constructed the antibiotic resistome during manure composting. However, the residues of ARGs and pathogens in compost products may still pose risks to human and crops after fertilization. Keywords: Antibiotic resistance genes, Livestock manure, Composting, Microbial community, Pathogens, Antibiotic resistomehttp://www.sciencedirect.com/science/article/pii/S0160412019333057 |