CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway
Zhongxin Feng,1,2,* Qi Chen,2,* Mingqiang Ren,2 Zuguo Tian,2 Yuping Gong1 1Department of Hematology, West China School of Medicine/West China Hospital, Sichuan University, Chengdu, China; 2Department of Hematology, Affiliated Hospital of Zunyi Medical University, Zunyi, China *These authors contri...
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doaj-623fec9564e0414690a6ad539f4832002020-11-24T20:49:14ZengDove Medical PressOncoTargets and Therapy1178-69302019-04-01Volume 123011301745217CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathwayFeng ZChen QRen MTian ZGong YZhongxin Feng,1,2,* Qi Chen,2,* Mingqiang Ren,2 Zuguo Tian,2 Yuping Gong1 1Department of Hematology, West China School of Medicine/West China Hospital, Sichuan University, Chengdu, China; 2Department of Hematology, Affiliated Hospital of Zunyi Medical University, Zunyi, China *These authors contributed equally to this work Introduction: Acute myeloid leukemia (AML), the hematological malignant tumor with high mortality, is still difficult to treat. CD40L is a type II transmembrane protein, which has been reported to have the potential to inhibit growth of some cancer cells. Materials and methods: In order to determine the role of CD40L on AML-M5 cell line THP-1, we overexpressed CD40L in the cells using a lentiviral vector system (pHBLV-CMVIE-Zs Green-T2A-puro vector); overexpression was confirmed by the detection of green fluorescent protein and CD40L protein expression. Results: Cellular apoptosis, proliferation, and cycle assays showed that CD40L could promote the apoptosis of, suppress the proliferation of, and stimulate the arrest of the G1/S phase of THP-1 cells. Finally, the protein expression of P53, Bax/Bcl-2, cyclinD1, PCNA, PTEN, and p-Akt illustrated that CD40L may partly influence cell growth of THP-1 cells through those genes, which was confirmed by immunohistochemistry and a PI3K/Akt activator. Conclusion: Taken together, CD40L could inhibit cell growth of THP-1 cells through the PI3K/Akt pathway, indicating that the overexpression of CD40L may be a potential target to treat the AML-M5 disease. Keywords: CD40L, cell proliferation, AML-M5, P53, cyclinD1, PCNA, tumor suppressor, cell apoptosishttps://www.dovepress.com/cd40l-inhibits-cell-growth-of-thp-1-cells-by-suppressing-the-pi3kakt-p-peer-reviewed-article-OTTCD40Lcell proliferationAML-M5P53cyclinD1PCNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Feng Z Chen Q Ren M Tian Z Gong Y |
spellingShingle |
Feng Z Chen Q Ren M Tian Z Gong Y CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway OncoTargets and Therapy CD40L cell proliferation AML-M5 P53 cyclinD1 PCNA |
author_facet |
Feng Z Chen Q Ren M Tian Z Gong Y |
author_sort |
Feng Z |
title |
CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway |
title_short |
CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway |
title_full |
CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway |
title_fullStr |
CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway |
title_full_unstemmed |
CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway |
title_sort |
cd40l inhibits cell growth of thp-1 cells by suppressing the pi3k/akt pathway |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2019-04-01 |
description |
Zhongxin Feng,1,2,* Qi Chen,2,* Mingqiang Ren,2 Zuguo Tian,2 Yuping Gong1 1Department of Hematology, West China School of Medicine/West China Hospital, Sichuan University, Chengdu, China; 2Department of Hematology, Affiliated Hospital of Zunyi Medical University, Zunyi, China *These authors contributed equally to this work Introduction: Acute myeloid leukemia (AML), the hematological malignant tumor with high mortality, is still difficult to treat. CD40L is a type II transmembrane protein, which has been reported to have the potential to inhibit growth of some cancer cells. Materials and methods: In order to determine the role of CD40L on AML-M5 cell line THP-1, we overexpressed CD40L in the cells using a lentiviral vector system (pHBLV-CMVIE-Zs Green-T2A-puro vector); overexpression was confirmed by the detection of green fluorescent protein and CD40L protein expression. Results: Cellular apoptosis, proliferation, and cycle assays showed that CD40L could promote the apoptosis of, suppress the proliferation of, and stimulate the arrest of the G1/S phase of THP-1 cells. Finally, the protein expression of P53, Bax/Bcl-2, cyclinD1, PCNA, PTEN, and p-Akt illustrated that CD40L may partly influence cell growth of THP-1 cells through those genes, which was confirmed by immunohistochemistry and a PI3K/Akt activator. Conclusion: Taken together, CD40L could inhibit cell growth of THP-1 cells through the PI3K/Akt pathway, indicating that the overexpression of CD40L may be a potential target to treat the AML-M5 disease. Keywords: CD40L, cell proliferation, AML-M5, P53, cyclinD1, PCNA, tumor suppressor, cell apoptosis |
topic |
CD40L cell proliferation AML-M5 P53 cyclinD1 PCNA |
url |
https://www.dovepress.com/cd40l-inhibits-cell-growth-of-thp-1-cells-by-suppressing-the-pi3kakt-p-peer-reviewed-article-OTT |
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