Aplicação de métodos de calibração multivariada para a determinação simultânea de riboflavina (VB2), tiamina (VB1), piridoxina (VB6) e nicotinamida (VPP) UV spectrophotrometry and chemometrics methods for simultaneous determinations of riboflavin (VB2), thiamine (VB1), pyridoxine (VB6) and nicotinamide (VPP)

• Main Authors: Rosângela C. Barthus, Luiz H. Mazo, Ronei J. Poppi
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Química 2007-01-01
• Series: Química Nova
• Subjects: water-soluble vitamins; UV spectrophotometry; multivariate calibration
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422007000700026
• ISSN: 0100-4042; 1678-7064

<abstract language="eng">In this work, the artificial neural networks (ANN) and partial least squares (PLS) regression were applied to UV spectral data for quantitative determination of thiamin hydrochloride (VB1), riboflavin phosphate (VB2), pyridoxine hydrochloride (VB6) and nicotinamide (VPP) in pharmaceutical samples. For calibration purposes, commercial samples in 0.2 mol L-1 acetate buffer (pH 4.0) were employed as standards. The concentration ranges used in the calibration step were: 0.1 - 7.5 mg L-1 for VB1, 0.1 - 3.0 mg L-1 for VB2, 0.1 - 3.0 mg L-1 for VB6 and 0.4 - 30.0 mg L-1 for VPP. From the results it is possible to verify that both methods can be successfully applied for these determinations. The similar error values were obtained by using neural network or PLS methods. The proposed methodology is simple, rapid and can be easily used in quality control laboratories.