Genomic variation in captive deer mouse (Peromyscus maniculatus) populations
Abstract Background Deer mice (genus Peromyscus) are the most common rodents in North America. Despite the availability of reference genomes for some species, a comprehensive database of polymorphisms, especially in those maintained as living stocks and distributed to academic investigators, is miss...
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doaj-624683376d8644fd929c9ec0c3cdc6252021-09-19T11:36:33ZengBMCBMC Genomics1471-21642021-09-0122111510.1186/s12864-021-07956-wGenomic variation in captive deer mouse (Peromyscus maniculatus) populationsMatthew D. Lucius0Hao Ji1Diego Altomare2Robert Doran3Ben Torkian4Amanda Havighorst5Vimala Kaza6Youwen Zhang7Alexander V. Gasparian8Joseph Magagnoli9Vijay Shankar10Michael Shtutman11Hippokratis Kiaris12Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaResearch Computing, Division of Information Technology, University of South CarolinaResearch Computing, Division of Information Technology, University of South CarolinaDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaPeromyscus Genetic Stock Center, University of South CarolinaDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaDepartment of Clinical Pharmacy and Outcomes Sciences, College of Pharmacy, University of South CarolinaCenter for Human Genetics, College of Science, Clemson UniversityDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South CarolinaAbstract Background Deer mice (genus Peromyscus) are the most common rodents in North America. Despite the availability of reference genomes for some species, a comprehensive database of polymorphisms, especially in those maintained as living stocks and distributed to academic investigators, is missing. In the present study we surveyed two populations of P. maniculatus that are maintained at the Peromyscus Genetic Stock Center (PGSC) for polymorphisms across their 2.5 × 109 bp genome. Results High density of variation was identified, corresponding to one SNP every 55 bp for the high altitude stock (SM2) or 207 bp for the low altitude stock (BW) using snpEff (v4.3). Indels were detected every 1157 bp for BW or 311 bp for SM2. The average Watterson estimator for the BW and SM2 populations is 248813.70388 and 869071.7671 respectively. Some differences in the distribution of missense, nonsense and silent mutations were identified between the stocks, as well as polymorphisms in genes associated with inflammation (NFATC2), hypoxia (HIF1a) and cholesterol metabolism (INSIG1) and may possess value in modeling pathology. Conclusions This genomic resource, in combination with the availability of P. maniculatus from the PGSC, is expected to promote genetic and genomic studies with this animal model.https://doi.org/10.1186/s12864-021-07956-wP. maniculatus bairdiiP. maniculatus sonoriensisSingle nucleotide polymorphismIndelWhole genome sequencingGenomic variation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthew D. Lucius Hao Ji Diego Altomare Robert Doran Ben Torkian Amanda Havighorst Vimala Kaza Youwen Zhang Alexander V. Gasparian Joseph Magagnoli Vijay Shankar Michael Shtutman Hippokratis Kiaris |
spellingShingle |
Matthew D. Lucius Hao Ji Diego Altomare Robert Doran Ben Torkian Amanda Havighorst Vimala Kaza Youwen Zhang Alexander V. Gasparian Joseph Magagnoli Vijay Shankar Michael Shtutman Hippokratis Kiaris Genomic variation in captive deer mouse (Peromyscus maniculatus) populations BMC Genomics P. maniculatus bairdii P. maniculatus sonoriensis Single nucleotide polymorphism Indel Whole genome sequencing Genomic variation |
author_facet |
Matthew D. Lucius Hao Ji Diego Altomare Robert Doran Ben Torkian Amanda Havighorst Vimala Kaza Youwen Zhang Alexander V. Gasparian Joseph Magagnoli Vijay Shankar Michael Shtutman Hippokratis Kiaris |
author_sort |
Matthew D. Lucius |
title |
Genomic variation in captive deer mouse (Peromyscus maniculatus) populations |
title_short |
Genomic variation in captive deer mouse (Peromyscus maniculatus) populations |
title_full |
Genomic variation in captive deer mouse (Peromyscus maniculatus) populations |
title_fullStr |
Genomic variation in captive deer mouse (Peromyscus maniculatus) populations |
title_full_unstemmed |
Genomic variation in captive deer mouse (Peromyscus maniculatus) populations |
title_sort |
genomic variation in captive deer mouse (peromyscus maniculatus) populations |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2021-09-01 |
description |
Abstract Background Deer mice (genus Peromyscus) are the most common rodents in North America. Despite the availability of reference genomes for some species, a comprehensive database of polymorphisms, especially in those maintained as living stocks and distributed to academic investigators, is missing. In the present study we surveyed two populations of P. maniculatus that are maintained at the Peromyscus Genetic Stock Center (PGSC) for polymorphisms across their 2.5 × 109 bp genome. Results High density of variation was identified, corresponding to one SNP every 55 bp for the high altitude stock (SM2) or 207 bp for the low altitude stock (BW) using snpEff (v4.3). Indels were detected every 1157 bp for BW or 311 bp for SM2. The average Watterson estimator for the BW and SM2 populations is 248813.70388 and 869071.7671 respectively. Some differences in the distribution of missense, nonsense and silent mutations were identified between the stocks, as well as polymorphisms in genes associated with inflammation (NFATC2), hypoxia (HIF1a) and cholesterol metabolism (INSIG1) and may possess value in modeling pathology. Conclusions This genomic resource, in combination with the availability of P. maniculatus from the PGSC, is expected to promote genetic and genomic studies with this animal model. |
topic |
P. maniculatus bairdii P. maniculatus sonoriensis Single nucleotide polymorphism Indel Whole genome sequencing Genomic variation |
url |
https://doi.org/10.1186/s12864-021-07956-w |
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