Low intensity exercise training improves skeletal muscle regeneration potential
Purpose: The aim of this study was to determine whether 12 days of low-to-moderate exercise training at low altitude (598 m a.s.l.) improves skeletal muscle regeneration in sedentary adult women.Methods: Satellite cells were obtained from the vastus lateralis skeletal muscle of seven women before an...
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doaj-624ff893588441a6b1ac6ed107497c532020-11-25T00:30:06ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2015-12-01610.3389/fphys.2015.00399169745Low intensity exercise training improves skeletal muscle regeneration potentialTiziana ePietrangelo0Tiziana ePietrangelo1Tiziana ePietrangelo2Tiziana ePietrangelo3Ester Sara Di Filippo4Ester Sara Di Filippo5Ester Sara Di Filippo6Rosa eMancinelli7Rosa eMancinelli8Rosa eMancinelli9Christian eDoria10Christian eDoria11Christian eDoria12Alessio eRotini13Alessio eRotini14Giorgio eFanò15Giorgio eFanò16Giorgio eFanò17Stefania eFulle18Stefania eFulle19Stefania eFulle20Stefania eFulle21University G. d'Annunzio Chieti-PescaraCentre for Aging Sciences (Ce.S.I.), d’Annunzio FoundationInteruniversity Institute of MyologyLaboratory of Functional Evaluation, ‘G. d’Annunzio’ University of Chieti–PescaraUniversity G. d'Annunzio Chieti-PescaraCentre for Aging Sciences (Ce.S.I.), d’Annunzio FoundationInteruniversity Institute of MyologyUniversity G. d'Annunzio Chieti-PescaraCentre for Aging Sciences (Ce.S.I.), d’Annunzio FoundationInteruniversity Institute of MyologyUniversity G. d'Annunzio Chieti-PescaraInteruniversity Institute of MyologyLaboratory of Functional Evaluation, ‘G. d’Annunzio’ University of Chieti–PescaraUniversity G. d'Annunzio Chieti-PescaraInteruniversity Institute of MyologyCentre for Aging Sciences (Ce.S.I.), d’Annunzio FoundationInteruniversity Institute of MyologyLaboratory of Functional Evaluation, ‘G. d’Annunzio’ University of Chieti–PescaraUniversity G. d'Annunzio Chieti-PescaraCentre for Aging Sciences (Ce.S.I.), d’Annunzio FoundationInteruniversity Institute of MyologyLaboratory of Functional Evaluation, ‘G. d’Annunzio’ University of Chieti–PescaraPurpose: The aim of this study was to determine whether 12 days of low-to-moderate exercise training at low altitude (598 m a.s.l.) improves skeletal muscle regeneration in sedentary adult women.Methods: Satellite cells were obtained from the vastus lateralis skeletal muscle of seven women before and after this exercise training at low altitude. They were investigated for differentiation aspects, superoxide anion production, antioxidant enzymes, mitochondrial potential variation after a depolarizing insult, intracellular Ca2+ concentrations, and micro (mi)RNA expression (miR-1, miR-133, miR-206).Results: In these myogenic populations of adult stem cells, those obtained after exercise training, showed increased Fusion Index and intracellular Ca2+ concentrations. This exercise training also generally reduced superoxide anion production in cells (by 12% to 67%), although not in two women, where there was an increase of ~15% along with a reduced superoxide dismutase activity. miRNA expression showed an exercise-induced epigenetic transcription profile that was specific according to the reduced or increased superoxide anion production of the cells. Conclusions: The present study shows that low-to-moderate exercise training at low altitude improves the regenerative capacity of skeletal muscle in adult women. The differentiation of cells was favored by increased intracellular calcium concentration and increased the fusion index. This low-to-moderate training at low altitude also depicted the epigenetic signature of cells.http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00399/fullWomenmiRNAexercise trainingsatellite cellsSuperoxide anionOxidative status |
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English |
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Article |
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DOAJ |
author |
Tiziana ePietrangelo Tiziana ePietrangelo Tiziana ePietrangelo Tiziana ePietrangelo Ester Sara Di Filippo Ester Sara Di Filippo Ester Sara Di Filippo Rosa eMancinelli Rosa eMancinelli Rosa eMancinelli Christian eDoria Christian eDoria Christian eDoria Alessio eRotini Alessio eRotini Giorgio eFanò Giorgio eFanò Giorgio eFanò Stefania eFulle Stefania eFulle Stefania eFulle Stefania eFulle |
spellingShingle |
Tiziana ePietrangelo Tiziana ePietrangelo Tiziana ePietrangelo Tiziana ePietrangelo Ester Sara Di Filippo Ester Sara Di Filippo Ester Sara Di Filippo Rosa eMancinelli Rosa eMancinelli Rosa eMancinelli Christian eDoria Christian eDoria Christian eDoria Alessio eRotini Alessio eRotini Giorgio eFanò Giorgio eFanò Giorgio eFanò Stefania eFulle Stefania eFulle Stefania eFulle Stefania eFulle Low intensity exercise training improves skeletal muscle regeneration potential Frontiers in Physiology Women miRNA exercise training satellite cells Superoxide anion Oxidative status |
author_facet |
Tiziana ePietrangelo Tiziana ePietrangelo Tiziana ePietrangelo Tiziana ePietrangelo Ester Sara Di Filippo Ester Sara Di Filippo Ester Sara Di Filippo Rosa eMancinelli Rosa eMancinelli Rosa eMancinelli Christian eDoria Christian eDoria Christian eDoria Alessio eRotini Alessio eRotini Giorgio eFanò Giorgio eFanò Giorgio eFanò Stefania eFulle Stefania eFulle Stefania eFulle Stefania eFulle |
author_sort |
Tiziana ePietrangelo |
title |
Low intensity exercise training improves skeletal muscle regeneration potential |
title_short |
Low intensity exercise training improves skeletal muscle regeneration potential |
title_full |
Low intensity exercise training improves skeletal muscle regeneration potential |
title_fullStr |
Low intensity exercise training improves skeletal muscle regeneration potential |
title_full_unstemmed |
Low intensity exercise training improves skeletal muscle regeneration potential |
title_sort |
low intensity exercise training improves skeletal muscle regeneration potential |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2015-12-01 |
description |
Purpose: The aim of this study was to determine whether 12 days of low-to-moderate exercise training at low altitude (598 m a.s.l.) improves skeletal muscle regeneration in sedentary adult women.Methods: Satellite cells were obtained from the vastus lateralis skeletal muscle of seven women before and after this exercise training at low altitude. They were investigated for differentiation aspects, superoxide anion production, antioxidant enzymes, mitochondrial potential variation after a depolarizing insult, intracellular Ca2+ concentrations, and micro (mi)RNA expression (miR-1, miR-133, miR-206).Results: In these myogenic populations of adult stem cells, those obtained after exercise training, showed increased Fusion Index and intracellular Ca2+ concentrations. This exercise training also generally reduced superoxide anion production in cells (by 12% to 67%), although not in two women, where there was an increase of ~15% along with a reduced superoxide dismutase activity. miRNA expression showed an exercise-induced epigenetic transcription profile that was specific according to the reduced or increased superoxide anion production of the cells. Conclusions: The present study shows that low-to-moderate exercise training at low altitude improves the regenerative capacity of skeletal muscle in adult women. The differentiation of cells was favored by increased intracellular calcium concentration and increased the fusion index. This low-to-moderate training at low altitude also depicted the epigenetic signature of cells. |
topic |
Women miRNA exercise training satellite cells Superoxide anion Oxidative status |
url |
http://journal.frontiersin.org/Journal/10.3389/fphys.2015.00399/full |
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