Mechanics and dynamics of translocating MreB filaments on curved membranes
MreB is an actin homolog that is essential for coordinating the cell wall synthesis required for the rod shape of many bacteria. Previously we have shown that filaments of MreB bind to the curved membranes of bacteria and translocate in directions determined by principal membrane curvatures to creat...
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doaj-6254eab6aad74167ae27e439032e3bd62021-05-05T17:25:14ZengeLife Sciences Publications LtdeLife2050-084X2019-02-01810.7554/eLife.40472Mechanics and dynamics of translocating MreB filaments on curved membranesFelix Wong0https://orcid.org/0000-0002-2309-8835Ethan C Garner1https://orcid.org/0000-0003-0141-3555Ariel Amir2https://orcid.org/0000-0003-2611-0139John A Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, United StatesDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, United States; Center for Systems Biology, Harvard University, Cambridge, United StatesJohn A Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, United StatesMreB is an actin homolog that is essential for coordinating the cell wall synthesis required for the rod shape of many bacteria. Previously we have shown that filaments of MreB bind to the curved membranes of bacteria and translocate in directions determined by principal membrane curvatures to create and reinforce the rod shape (Hussain et al., 2018). Here, in order to understand how MreB filament dynamics affects their cellular distribution, we model how MreB filaments bind and translocate on membranes with different geometries. We find that it is both energetically favorable and robust for filaments to bind and orient along directions of largest membrane curvature. Furthermore, significant localization to different membrane regions results from processive MreB motion in various geometries. These results demonstrate that the in vivo localization of MreB observed in many different experiments, including those examining negative Gaussian curvature, can arise from translocation dynamics alone.https://elifesciences.org/articles/40472MreBmorphogenesisshapemembranecurvaturelocalization |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Felix Wong Ethan C Garner Ariel Amir |
spellingShingle |
Felix Wong Ethan C Garner Ariel Amir Mechanics and dynamics of translocating MreB filaments on curved membranes eLife MreB morphogenesis shape membrane curvature localization |
author_facet |
Felix Wong Ethan C Garner Ariel Amir |
author_sort |
Felix Wong |
title |
Mechanics and dynamics of translocating MreB filaments on curved membranes |
title_short |
Mechanics and dynamics of translocating MreB filaments on curved membranes |
title_full |
Mechanics and dynamics of translocating MreB filaments on curved membranes |
title_fullStr |
Mechanics and dynamics of translocating MreB filaments on curved membranes |
title_full_unstemmed |
Mechanics and dynamics of translocating MreB filaments on curved membranes |
title_sort |
mechanics and dynamics of translocating mreb filaments on curved membranes |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2019-02-01 |
description |
MreB is an actin homolog that is essential for coordinating the cell wall synthesis required for the rod shape of many bacteria. Previously we have shown that filaments of MreB bind to the curved membranes of bacteria and translocate in directions determined by principal membrane curvatures to create and reinforce the rod shape (Hussain et al., 2018). Here, in order to understand how MreB filament dynamics affects their cellular distribution, we model how MreB filaments bind and translocate on membranes with different geometries. We find that it is both energetically favorable and robust for filaments to bind and orient along directions of largest membrane curvature. Furthermore, significant localization to different membrane regions results from processive MreB motion in various geometries. These results demonstrate that the in vivo localization of MreB observed in many different experiments, including those examining negative Gaussian curvature, can arise from translocation dynamics alone. |
topic |
MreB morphogenesis shape membrane curvature localization |
url |
https://elifesciences.org/articles/40472 |
work_keys_str_mv |
AT felixwong mechanicsanddynamicsoftranslocatingmrebfilamentsoncurvedmembranes AT ethancgarner mechanicsanddynamicsoftranslocatingmrebfilamentsoncurvedmembranes AT arielamir mechanicsanddynamicsoftranslocatingmrebfilamentsoncurvedmembranes |
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1721459312758358016 |