The genetic variants at the HLA-DRB1 gene are associated with primary IgA nephropathy in Han Chinese

• Main Authors: Jiyun Yang, Guisen Li, Li Zhu, Yi Shi, Jicheng Lv, Fang Lu, Xiaoqi Liu, Shi Ma, Cheng Jing, Ying Lin, Haiyan Wang, Li Wang, Hong Zhang, Zhenglin Yang
• Format: Article
• Language: English
• Published: BMC 2012-05-01
• Series: BMC Medical Genetics
• Subjects: IgA nephropathy; HLA-DRB1; Association study
• Online Access: http://www.biomedcentral.com/1471-2350/13/33

<p>Abstract</p> <p>Background</p> <p>Immunoglobulin A nephropathy (IgAN), an immune-complex-mediated glomerulonephritis defined immunohistologically by the presence of glomerular IgA deposits, is the most common primary glomerular disease worldwide and a significant cause of end-stage renal disease. Familial clustering of patients with IgAN suggests a genetic predisposition.</p> <p>Methods</p> <p>In this study, 192 patients with IgAN and 192 normal controls in the Sichuan cohort and 935 patients with IgAN and 2,103 normal controls in the Beijing cohort were investigated. HLA-DRB1*01–DRB1*10 specificities were genotyped by the PCR–SSP technique in both cohorts. Based on the HLA-DRB1*04-positive results, the subtypes of HLA-DRB1*04 were analyzed using sequencing-based typing (SBT) in 291 IgAN cases and 420 matched controls.</p> <p>Results</p> <p>The frequency of HLA-DRB1*04 in the IgAN group was significantly higher than that in the control group (0.129 vs. 0.092, <it>P</it> = 8.29 × 10^-5, odds ratio (OR) =1.381, 95% confidence interval (CI) 1.178–1.619). Other alleles at the HLA-DRB1 locus were observed with no significant differences between the case and control groups. The dominant alleles of the HLA-DRB1*04 subtypes were DRB1*0405 in both cohorts. The frequencies of HLA-DRB1*0405 and 0403 were significantly increased in the patients compared to healthy subjects.</p> <p>Conclusion</p> <p>HLA-DRB1*04 was significantly associated with primary IgAN in Chinese population. This result implies that HLA-DRB1 gene plays a major role in primary IgAN.</p>