Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine

The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H...

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Main Authors: Quyen Thi Nguyen, Chaewon Kwak, Wang Sik Lee, Jaemoo Kim, Jinyoung Jeong, Moon Hee Sung, Jihyun Yang, Haryoung Poo
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-07-01
Series:Frontiers in Immunology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.01604/full
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spelling doaj-62596beb0ba74d18a775f141db1323d92020-11-25T01:56:16ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-07-011010.3389/fimmu.2019.01604465567Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 VaccineQuyen Thi Nguyen0Quyen Thi Nguyen1Chaewon Kwak2Chaewon Kwak3Wang Sik Lee4Wang Sik Lee5Jaemoo Kim6Jaemoo Kim7Jinyoung Jeong8Jinyoung Jeong9Moon Hee Sung10Jihyun Yang11Haryoung Poo12Haryoung Poo13Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaDepartment of Bio and Nanochemistry, Kookmin University, Seoul, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaThe use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases.https://www.frontiersin.org/article/10.3389/fimmu.2019.01604/fullvaccine adjuvantinfluenza virusefficacycross-protectionantibody-dependent cellular cytotoxicitycytotoxic T lymphocyte activity
collection DOAJ
language English
format Article
sources DOAJ
author Quyen Thi Nguyen
Quyen Thi Nguyen
Chaewon Kwak
Chaewon Kwak
Wang Sik Lee
Wang Sik Lee
Jaemoo Kim
Jaemoo Kim
Jinyoung Jeong
Jinyoung Jeong
Moon Hee Sung
Jihyun Yang
Haryoung Poo
Haryoung Poo
spellingShingle Quyen Thi Nguyen
Quyen Thi Nguyen
Chaewon Kwak
Chaewon Kwak
Wang Sik Lee
Wang Sik Lee
Jaemoo Kim
Jaemoo Kim
Jinyoung Jeong
Jinyoung Jeong
Moon Hee Sung
Jihyun Yang
Haryoung Poo
Haryoung Poo
Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
Frontiers in Immunology
vaccine adjuvant
influenza virus
efficacy
cross-protection
antibody-dependent cellular cytotoxicity
cytotoxic T lymphocyte activity
author_facet Quyen Thi Nguyen
Quyen Thi Nguyen
Chaewon Kwak
Chaewon Kwak
Wang Sik Lee
Wang Sik Lee
Jaemoo Kim
Jaemoo Kim
Jinyoung Jeong
Jinyoung Jeong
Moon Hee Sung
Jihyun Yang
Haryoung Poo
Haryoung Poo
author_sort Quyen Thi Nguyen
title Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
title_short Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
title_full Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
title_fullStr Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
title_full_unstemmed Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
title_sort poly-γ-glutamic acid complexed with alum induces cross-protective immunity of pandemic h1n1 vaccine
publisher Frontiers Media S.A.
series Frontiers in Immunology
issn 1664-3224
publishDate 2019-07-01
description The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases.
topic vaccine adjuvant
influenza virus
efficacy
cross-protection
antibody-dependent cellular cytotoxicity
cytotoxic T lymphocyte activity
url https://www.frontiersin.org/article/10.3389/fimmu.2019.01604/full
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