Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine
The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H...
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doaj-62596beb0ba74d18a775f141db1323d92020-11-25T01:56:16ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-07-011010.3389/fimmu.2019.01604465567Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 VaccineQuyen Thi Nguyen0Quyen Thi Nguyen1Chaewon Kwak2Chaewon Kwak3Wang Sik Lee4Wang Sik Lee5Jaemoo Kim6Jaemoo Kim7Jinyoung Jeong8Jinyoung Jeong9Moon Hee Sung10Jihyun Yang11Haryoung Poo12Haryoung Poo13Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaDepartment of Bio and Nanochemistry, Kookmin University, Seoul, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaInfectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South KoreaDepartment of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, South KoreaThe use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases.https://www.frontiersin.org/article/10.3389/fimmu.2019.01604/fullvaccine adjuvantinfluenza virusefficacycross-protectionantibody-dependent cellular cytotoxicitycytotoxic T lymphocyte activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Quyen Thi Nguyen Quyen Thi Nguyen Chaewon Kwak Chaewon Kwak Wang Sik Lee Wang Sik Lee Jaemoo Kim Jaemoo Kim Jinyoung Jeong Jinyoung Jeong Moon Hee Sung Jihyun Yang Haryoung Poo Haryoung Poo |
spellingShingle |
Quyen Thi Nguyen Quyen Thi Nguyen Chaewon Kwak Chaewon Kwak Wang Sik Lee Wang Sik Lee Jaemoo Kim Jaemoo Kim Jinyoung Jeong Jinyoung Jeong Moon Hee Sung Jihyun Yang Haryoung Poo Haryoung Poo Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine Frontiers in Immunology vaccine adjuvant influenza virus efficacy cross-protection antibody-dependent cellular cytotoxicity cytotoxic T lymphocyte activity |
author_facet |
Quyen Thi Nguyen Quyen Thi Nguyen Chaewon Kwak Chaewon Kwak Wang Sik Lee Wang Sik Lee Jaemoo Kim Jaemoo Kim Jinyoung Jeong Jinyoung Jeong Moon Hee Sung Jihyun Yang Haryoung Poo Haryoung Poo |
author_sort |
Quyen Thi Nguyen |
title |
Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine |
title_short |
Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine |
title_full |
Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine |
title_fullStr |
Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine |
title_full_unstemmed |
Poly-γ-Glutamic Acid Complexed With Alum Induces Cross-Protective Immunity of Pandemic H1N1 Vaccine |
title_sort |
poly-γ-glutamic acid complexed with alum induces cross-protective immunity of pandemic h1n1 vaccine |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-07-01 |
description |
The use of a good vaccine adjuvant may induce a higher immunogenicity profile of vaccine antigens. Here, we developed a new adjuvant by combining poly-γ-glutamic acid (γ-PGA) with alum (PGA/Alum) and investigated its ability to enhance the immunogenicity and the cross-reactive efficacy of pandemic H1N1 (pH1N1) influenza vaccine antigen. PGA/Alum enhanced antigen delivery to draining lymph nodes and antigen-specific immunogenicity in mice using OVA as a model antigen. It also greatly increased OVA-specific antibody production, cytotoxic T lymphocyte (CTL) activity, and antibody-dependent cellular cytotoxicity (ADCC). These abilities of PGA/Alum improved the protective efficacy of pH1N1 vaccine antigen by increasing hemagglutination-inhibition titers, enhancing ADCC and CTL activity, and speeding viral clearance following homologous viral challenge. Importantly, the cross-protective efficacy of pH1N1 vaccine against heterologous viruses [A/Puerto Rico/8/34 (H1N1) and A/Hong Kong/1/1968 (H3N2)] was significantly enhanced by PGA/Alum, and cross-reactive ADCC and CTL activities were observed. Together, our results strongly suggest that PGA/Alum may be a promising vaccine adjuvant for preventing influenza and other infectious diseases. |
topic |
vaccine adjuvant influenza virus efficacy cross-protection antibody-dependent cellular cytotoxicity cytotoxic T lymphocyte activity |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01604/full |
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