Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine
Securigera securidaca (L.) Degen & Dorfl grows in different parts of Iran. The seeds of the species are used in Iranian folk medicine as an anti-diabetic agent. Many studies have established hypoglycemic effects of amino acids and in the present investigation, amino acids of Securigera securidac...
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Iranian Society of Pharmacognosy
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doaj-6280439aaecd434db83f68210e4529512020-11-24T21:34:33ZengIranian Society of PharmacognosyResearch Journal of Pharmacognosy2345-44582345-59772014-01-011123263905Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicineS.E. Sadat-Ebrahimi0M. Hassanpoor Mir1G.R. Amin2H. Hajimehdipoor3Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.Department of pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Securigera securidaca (L.) Degen & Dorfl grows in different parts of Iran. The seeds of the species are used in Iranian folk medicine as an anti-diabetic agent. Many studies have established hypoglycemic effects of amino acids and in the present investigation, amino acids of Securigera securidaca seeds have been evaluated. The ground seeds were extracted using petroleum ether, hot ethanol and ethanol 50%, respectively. ethanol 50% extract was chromatographed over cation exchanging resin and the resulting amino acid fraction was subjected to HPLC after OPA derivatization and the amino acids were identified by comparing to standards. The results evidenced the presence of 19 amino acids in the plant extract including alanine, arginine, asparagine, aspartic acid, citrulline, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine and valine. Considering the role of some amino acids in diabetes the above amino acids could be noted as hypoglycemic agents of the plant seeds but further studies are necessary.http://www.rjpharmacognosy.ir/pdf_3905_bbac27d5aca927737db112abc9190b3a.htmlAmino acidHPLCIon Exchange ChromatographySecurigera securidaca |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S.E. Sadat-Ebrahimi M. Hassanpoor Mir G.R. Amin H. Hajimehdipoor |
spellingShingle |
S.E. Sadat-Ebrahimi M. Hassanpoor Mir G.R. Amin H. Hajimehdipoor Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine Research Journal of Pharmacognosy Amino acid HPLC Ion Exchange Chromatography Securigera securidaca |
author_facet |
S.E. Sadat-Ebrahimi M. Hassanpoor Mir G.R. Amin H. Hajimehdipoor |
author_sort |
S.E. Sadat-Ebrahimi |
title |
Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine |
title_short |
Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine |
title_full |
Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine |
title_fullStr |
Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine |
title_full_unstemmed |
Identification of amino acids in Securigera securidaca, a popular medicinal herb in Iranian folk medicine |
title_sort |
identification of amino acids in securigera securidaca, a popular medicinal herb in iranian folk medicine |
publisher |
Iranian Society of Pharmacognosy |
series |
Research Journal of Pharmacognosy |
issn |
2345-4458 2345-5977 |
publishDate |
2014-01-01 |
description |
Securigera securidaca (L.) Degen & Dorfl grows in different parts of Iran. The seeds of the species are used in Iranian folk medicine as an anti-diabetic agent. Many studies have established hypoglycemic effects of amino acids and in the present investigation, amino acids of Securigera securidaca seeds have been evaluated. The ground seeds were extracted using petroleum ether, hot ethanol and ethanol 50%, respectively. ethanol 50% extract was chromatographed over cation exchanging resin and the resulting amino acid fraction was subjected to HPLC after OPA derivatization and the amino acids were identified by comparing to standards. The results evidenced the presence of 19 amino acids in the plant extract including alanine, arginine, asparagine, aspartic acid, citrulline, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tyrosine and valine. Considering the role of some amino acids in diabetes the above amino acids could be noted as hypoglycemic agents of the plant seeds but further studies are necessary. |
topic |
Amino acid HPLC Ion Exchange Chromatography Securigera securidaca |
url |
http://www.rjpharmacognosy.ir/pdf_3905_bbac27d5aca927737db112abc9190b3a.html |
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