New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles
Wastewater from hospitals and facilities that receive patients infected with contagious microorganisms has dense concentrations of these pathogens, which may represent a threat to public health. Thus, proper wastewater treatment to remove these contaminants before discharging to the sewage system ha...
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doaj-6291e82126f84b42bf842dbcdc7c187c2021-10-02T19:11:51ZengDe GruyterGreen Processing and Synthesis2191-95422191-95502016-10-015547347810.1515/gps-2016-0024New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticlesNguyen Dao Q.0Duong Phuong T.1Nguyen Hieu M.2Nam Nguyen Hoang3Luong Nguyen Hoang4Pham Yen5Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Hanoi, Viet NamFaculty of Biology, VNU University of Science, 334 Nguyen Trai, Hanoi, Viet NamNano and Energy Center, VNU University of Science, 334 Nguyen Trai, Hanoi, Viet NamNano and Energy Center, VNU University of Science, 334 Nguyen Trai, Hanoi, Viet NamNano and Energy Center, VNU University of Science, 334 Nguyen Trai, Hanoi, Viet NamKey Laboratory of Enzyme and Protein Technology, VNU University of Science, 334 Nguyen Trai, Hanoi, Viet NamWastewater from hospitals and facilities that receive patients infected with contagious microorganisms has dense concentrations of these pathogens, which may represent a threat to public health. Thus, proper wastewater treatment to remove these contaminants before discharging to the sewage system has been a great concern to the society. Aside from chemical and physical treatment, biological alternatives have been more widely studied and implemented for wastewater processing to avoid unwanted effects and to lower the costs. This study aimed to use lysing enzymes coupled to magnetic amino nanoparticles functionalized with specific antibodies (NP-NH2-antibody) to treat wastewater contaminated with targeted contagious pathogens, initially with Mycobacterium tuberculosis, a highly persistent bacterium. The generated complexes captured Mycobacterium bovis in Bacillus Calmette-Guérin (BCG; tuberculosis vaccine), which was subsequently lysed by lysozyme and lipase. The result showed that single complexes (functionalized with either enzyme or antibody) worked more efficiently than dual complexes (coupled to both proteins) and the complexes could be stored refrigerated stably for at least two months. The preliminary assessment of this method in treating wastewater model samples spiked with BCG vaccine showed evidence for the bacteria being captured and eliminated. Further optimization is necessary to implement these complexes in treating pathogen-contaminated wastewater.https://doi.org/10.1515/gps-2016-0024enzymesmycobacterium tuberculosisnanoparticleswastewater treatment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nguyen Dao Q. Duong Phuong T. Nguyen Hieu M. Nam Nguyen Hoang Luong Nguyen Hoang Pham Yen |
spellingShingle |
Nguyen Dao Q. Duong Phuong T. Nguyen Hieu M. Nam Nguyen Hoang Luong Nguyen Hoang Pham Yen New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles Green Processing and Synthesis enzymes mycobacterium tuberculosis nanoparticles wastewater treatment |
author_facet |
Nguyen Dao Q. Duong Phuong T. Nguyen Hieu M. Nam Nguyen Hoang Luong Nguyen Hoang Pham Yen |
author_sort |
Nguyen Dao Q. |
title |
New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles |
title_short |
New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles |
title_full |
New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles |
title_fullStr |
New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles |
title_full_unstemmed |
New biological treatment targeting Mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles |
title_sort |
new biological treatment targeting mycobacterium tuberculosis in contaminated wastewater using lysing enzymes coupled to magnetic nanoparticles |
publisher |
De Gruyter |
series |
Green Processing and Synthesis |
issn |
2191-9542 2191-9550 |
publishDate |
2016-10-01 |
description |
Wastewater from hospitals and facilities that receive patients infected with contagious microorganisms has dense concentrations of these pathogens, which may represent a threat to public health. Thus, proper wastewater treatment to remove these contaminants before discharging to the sewage system has been a great concern to the society. Aside from chemical and physical treatment, biological alternatives have been more widely studied and implemented for wastewater processing to avoid unwanted effects and to lower the costs. This study aimed to use lysing enzymes coupled to magnetic amino nanoparticles functionalized with specific antibodies (NP-NH2-antibody) to treat wastewater contaminated with targeted contagious pathogens, initially with Mycobacterium tuberculosis, a highly persistent bacterium. The generated complexes captured Mycobacterium bovis in Bacillus Calmette-Guérin (BCG; tuberculosis vaccine), which was subsequently lysed by lysozyme and lipase. The result showed that single complexes (functionalized with either enzyme or antibody) worked more efficiently than dual complexes (coupled to both proteins) and the complexes could be stored refrigerated stably for at least two months. The preliminary assessment of this method in treating wastewater model samples spiked with BCG vaccine showed evidence for the bacteria being captured and eliminated. Further optimization is necessary to implement these complexes in treating pathogen-contaminated wastewater. |
topic |
enzymes mycobacterium tuberculosis nanoparticles wastewater treatment |
url |
https://doi.org/10.1515/gps-2016-0024 |
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