BIOTRANSFORMATION OF METRONIDAZOLE BY CUNNINGHAMELLA ELEGANS ATCC 9245

• Main Authors: Julia Medeiros Sorrentino, Rafaela Martins Sponchiado, Natália Olegário dos Santos, Sendy Salles Oliveira, Karina Galle, Cassia Virginia Garcia, Alexandre Meneghello Fuentefria, Martin Steppe
• Format: Article
• Language: English
• Published: Universidade Federal do Rio Grande do Sul 2019-07-01
• Series: Drug Analytical Research
• Subjects: biotransformation; hplc; metabolites; metronidazole
• Online Access: https://seer.ufrgs.br/dar/article/view/94032/53386

Drug biotransformation studies appear as an alternative to pharmacological studies of metabolites, development of new drug candidates with reduced investment as well as the most efficient production of chemical structures involves and drug quality control studies. A wide range of reactions in biotransformations process is catalyzed by microorganisms. Fungi can be considered as a promising source of new biotransformation reactions. The aim of this study was to evaluate the capacity of metronidazole biotransformation through the filamentous fungus Cunninghamella elegans ATCC 9245. The monitoring of metabolite formation was performed by high-performance liquid chromatography (HPLC) coupled to ultraviolet (UV) spectrophotometry. The results of the biotransformation of metronidazole showed drug consumption in culture and the formation of four new chromatographic peaks of chemical structures not elucidated. The method showed it became linear over 10-70 μg/mL (r = 0.999953). Accuracy, precision and stability studies agree with international guidelines. Results are consistent in accordance with the principles of green chemistry as the experimental conditions had a low environmental impact, and few solvents use.