Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster
Hibernating animals have been used as models to study several aspects of the plastic changes that occur in the metabolism and physiology of neurons. These models are also of interest in the study of Alzheimer’s disease because the microtubule-associated protein tau is hyperphosphorylated during the...
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doaj-62a143bfc207445cbf0c04fdc1d56c452020-11-24T22:26:03ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292015-12-01910.3389/fnana.2015.00157168867Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamsterAlejandro eAntón0Alejandro eAntón1Gonzalo eLeon-Espinosa2Gonzalo eLeon-Espinosa3Javier eDeFelipe4Javier eDeFelipe5Alberto eMunoz6Alberto eMunoz7Alberto eMunoz8Politecnica University, MadridCajal Institute, CSICPolitecnica University, MadridCajal Institute, CSICPolitecnica University, MadridCajal Institute, CSICPolitecnica University, MadridCajal Institute, CSICFac. Biology, Complutense UniversityHibernating animals have been used as models to study several aspects of the plastic changes that occur in the metabolism and physiology of neurons. These models are also of interest in the study of Alzheimer’s disease because the microtubule-associated protein tau is hyperphosphorylated during the hibernation state known as torpor, similar to the pretangle stage of Alzheimer’s disease. Hibernating animals undergo torpor periods with drops in body temperature and metabolic rate, and a virtual cessation of neural activity. These processes are accompanied by morphological and neurochemical changes in neurons, which reverse a few hours after coming out of the torpor state. Since tau has been implicated in the structural regulation of the neuronal Golgi apparatus (GA) we have used Western Blot and immunocytochemistry to analyze whether the GA is modified in cortical neurons of the Syrian hamster at different hibernation stages. The results show that, during the hibernation cycle, the GA undergo important structural changes along with differential modifications in expression levels and distribution patterns of Golgi structural proteins. These changes were accompanied by significant transitory reductions in the volume and surface area of the GA elements during torpor and arousal stages as compared with euthermic animalshttp://journal.frontiersin.org/Journal/10.3389/fnana.2015.00157/fullHibernationpyramidal neuronGM130Golgin84MG160 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alejandro eAntón Alejandro eAntón Gonzalo eLeon-Espinosa Gonzalo eLeon-Espinosa Javier eDeFelipe Javier eDeFelipe Alberto eMunoz Alberto eMunoz Alberto eMunoz |
spellingShingle |
Alejandro eAntón Alejandro eAntón Gonzalo eLeon-Espinosa Gonzalo eLeon-Espinosa Javier eDeFelipe Javier eDeFelipe Alberto eMunoz Alberto eMunoz Alberto eMunoz Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster Frontiers in Neuroanatomy Hibernation pyramidal neuron GM130 Golgin84 MG160 |
author_facet |
Alejandro eAntón Alejandro eAntón Gonzalo eLeon-Espinosa Gonzalo eLeon-Espinosa Javier eDeFelipe Javier eDeFelipe Alberto eMunoz Alberto eMunoz Alberto eMunoz |
author_sort |
Alejandro eAntón |
title |
Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster |
title_short |
Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster |
title_full |
Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster |
title_fullStr |
Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster |
title_full_unstemmed |
Changes in the Golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster |
title_sort |
changes in the golgi apparatus of neocortical and hippocampal neurons in the hibernating hamster |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Neuroanatomy |
issn |
1662-5129 |
publishDate |
2015-12-01 |
description |
Hibernating animals have been used as models to study several aspects of the plastic changes that occur in the metabolism and physiology of neurons. These models are also of interest in the study of Alzheimer’s disease because the microtubule-associated protein tau is hyperphosphorylated during the hibernation state known as torpor, similar to the pretangle stage of Alzheimer’s disease. Hibernating animals undergo torpor periods with drops in body temperature and metabolic rate, and a virtual cessation of neural activity. These processes are accompanied by morphological and neurochemical changes in neurons, which reverse a few hours after coming out of the torpor state. Since tau has been implicated in the structural regulation of the neuronal Golgi apparatus (GA) we have used Western Blot and immunocytochemistry to analyze whether the GA is modified in cortical neurons of the Syrian hamster at different hibernation stages. The results show that, during the hibernation cycle, the GA undergo important structural changes along with differential modifications in expression levels and distribution patterns of Golgi structural proteins. These changes were accompanied by significant transitory reductions in the volume and surface area of the GA elements during torpor and arousal stages as compared with euthermic animals |
topic |
Hibernation pyramidal neuron GM130 Golgin84 MG160 |
url |
http://journal.frontiersin.org/Journal/10.3389/fnana.2015.00157/full |
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