Expression of microRNA-188 in gastric carcinoma and its effect on the proliferation and apoptosis of gastric cancer cells
Objective: To investigate the expression of microRNA-188 in tissues and blood of gastric cancer patients, and to study the effect of microRNA-188 on the proliferation and apoptosis of human gastric cancer cell line MGC-803 and its mechanism. Methods: Gastric cancer tissues and adjacent tissues we...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Editorial Board of Journal of Hainan Medical University
2019-06-01
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Series: | Journal of Hainan Medical University |
Subjects: | |
Online Access: | http://www.hnykdxxb.com/PDF/201911/5.pdf |
Summary: | Objective: To investigate the expression of microRNA-188 in tissues and blood of gastric
cancer patients, and to study the effect of microRNA-188 on the proliferation and apoptosis
of human gastric cancer cell line MGC-803 and its mechanism. Methods: Gastric cancer
tissues and adjacent tissues were collected from 10 patients with gastric cancer. Quantitative
real time PCR (qPCR) was used to detect the expression levels of microRNA-545, microRNA-
575, microRNA-1269, microRNA-99a, microRNA-452 and microRNA-188. The levels of
six microRNAs in blood samples were detected by qPCR in healthy volunteers and patients
with gastric cancer. The microRNA-188 mimics, microRNA-188 AMO and their NCs were
transfected into human gastric cancer cells MGC-803 using transfection reagent. After 24 h,
CCK-8 assay and TUNEL staining were used to detect the cell proliferation and apoptosis. The
website online was used to predict the target gene of microRNA-188. qRT-PCR and western
blot assays were used to determine whether microRNA-188 had a regulatory effect on SIX1.
Results: Compared with adjacent tissues, microRNA-188 was found to markedly decrease in
gastric cancer tissues. Compared with healthy volunteers, microRNA-188 was significantly
lower in the blood samples of gastric cancer patients. CCK-8 results showed that microRNA-
188 mimics significantly inhibited the proliferation of gastric cancer cells, while microRNA-
188 AMO could significantly increase the proliferation of gastric cancer cells. TUNEL
staining showed that microRNA-188 can promote the apoptosis of gastric cancer cells, and
its AMO can significantly inhibit the apoptosis of gastric cancer cells. qRT-PCR and western
blot experiments showed that microRNA-188 can significantly reduce the mRNA and protein
levels of its target SIX1. Conclusion: The expression level of microRNA-188 is significantly
decreased in the tissue and blood of patients with gastric cancer and microRNA-188 plays an
important role in regulating the proliferation and apoptosis of gastric cancer cells by inhibiting
SIX1. |
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ISSN: | 1007-1237 1007-1237 |