| Summary: | At present, bovine mastitis is one of the most costly diseases affecting animal health and welfare. Escherichia coli (E. coli) is considered to be one of the main pathogens causing mastitis with clinical signs in dairy cattle. However, the cure rate of E. coli mastitis is low, and the pathogenesis of E. coli mastitis is not completely known. In order to develop new strategies for the rapid detection of E. coli mastitis, a comprehensive molecular investigation of E. coli mastitis is necessary. Hence, this study integrated three microarray data sets to identify the potential key candidate genes in dairy cow in response to E. coli mastitis. Differentially expressed genes (DEGs) were screened in mammary gland tissues with live E. coli infection. Furthermore, the pathways enrichment of DEGs were analyzed, and the protein–protein interaction (PPI) network was performed. In total, 105 shared DEGs were identified from the three data sets. The DEGs were significantly enriched in biological processes mainly involved in immunity. The PPI network of DEGs was constructed with 102 nodes and 546 edges. The module with the highest score through MCODE analysis was filtered from PPI; 18 central node genes were identified. However, in addition to immune-related pathways, some of the 18 DEGs were involved in signaling pathways triggered by other diseases. Considering the specificity of biomarkers for rapid detection, IL8RB, CXCL6, and MMP9 were identified as the most potential biomarker for E. coli mastitis. In conclusion, the novel DEGs and pathways identified in this study can help to improve the diagnosis and treatment strategies for E. coli mastitis in cattle.
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