Scaling-up batch conditions for efficient sucrose hydrolysis catalyzed by an immobilized recombinant Pichia pastoris cells in a stirrer tank reactor

• Main Authors: Duniesky Martínez, Carmen Menéndez, Lázaro Hernández, Alina Sobrino, Luis E. Trujillo, Ivan Rodríguez, Enrique R. Pérez
• Format: Article
• Language: English
• Published: Elsevier 2017-01-01
• Series: Electronic Journal of Biotechnology
• Subjects: Bioreactor; Calcium alginate beads; Enzymatic biocatalyst; Immobilized biocatalyst; Industrial biotechnology; Invert sugar; Invertase; Kinetic model; Scale-up; Stirred tank reactor; Sucrose inversion
• Online Access: http://www.sciencedirect.com/science/article/pii/S0717345816301257

Background: Invert sugar is used greatly in food and pharmaceutical industries. This paper describes scaling-up batch conditions for sucrose inversion catalyzed by the recombinant Pichia pastoris BfrA4X whole cells expressing Thermotoga maritima invertase entrapped in calcium alginate beads. For the first time, we describe the application of a kinetic model to predict the fractional conversion expected during sucrose hydrolysis reaction in both, a model and a prototype bioreactor with 0.5- and 5-L working volume, respectively. Results: Different scaled-up criteria used to operate the 0.5-L bioreactor were analyzed to explore the invert sugar large scale production. After model inversion studies, a 5-L scaled-up reaction system was performed in a 7-L stirred reactor. Both scaled-up criteria, immobilized biocatalyst dosage and stirring speed, were analyzed in each type of bioreactors and the collected data were used to ensure an efficient scale-up of this biocatalyst. Conclusions: To date, there is not enough information to describe the large-scale production of invert sugar using different scaled-up criteria such as dose of immobilized biocatalyst and stirring speed effect on mass transfer. The present study results constitute a valuable tool to successfully carry out this type of high-scale operation for industrial purposes.