Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury
In this study effect of salvianolic acid B was observed on motor function recovery of rats with spinal cord injury. 50 rats were selected and after inducing SCI their recovery under controlled conditions was studied using Sal B and PBS (as control). Both compounds were introduced intraperitoneally i...
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Online Access: | http://dx.doi.org/10.1155/2014/931850 |
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doaj-62d0b4118bfc48b8859b6d431a56178f2020-11-24T22:19:19ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/931850931850Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord InjuryChong Xun0Yang Hu1Ming Lu2Shouyu Wang3Decheng Lv4Department of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, ChinaDepartment of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, ChinaDepartment of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, ChinaDepartment of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, ChinaDepartment of Orthopedic Surgery, First Affiliated Hospital of Dalian Medical University, Zhongshan Road, No. 222, Dalian, Liaoning 116000, ChinaIn this study effect of salvianolic acid B was observed on motor function recovery of rats with spinal cord injury. 50 rats were selected and after inducing SCI their recovery under controlled conditions was studied using Sal B and PBS (as control). Both compounds were introduced intraperitoneally in respective groups of traumatic rats at the same time intervals for 28 days. It was observed that Sal B introduced at 5 mg/kg/day resulted in better motor function recovery. BBB score was recorded which increased significantly along with the reduction in cavity area observed by bright field microscopy of tissues, that is, from 1 to 10 and from 0.20±0.05 mm2 to 0.10±0.03 mm2, in Sal B treated group, respectively, compared to PBS group. Statistical analysis was carried out using SPSS software (SPSS, Chicago, IL, USA), values were expressed as mean ± SEM, and P value <0.01 was considered significant. Effect of Sal B on expression of NF-kB p65 and IkBα was studied and OD values of densitometry of western blots were taken. MPO activity was also studied. It was observed that treatment of Sal B significantly reduced the expression of both compounds in Sal B treated group as compared to control group after 28 days of treatment.http://dx.doi.org/10.1155/2014/931850 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chong Xun Yang Hu Ming Lu Shouyu Wang Decheng Lv |
spellingShingle |
Chong Xun Yang Hu Ming Lu Shouyu Wang Decheng Lv Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury BioMed Research International |
author_facet |
Chong Xun Yang Hu Ming Lu Shouyu Wang Decheng Lv |
author_sort |
Chong Xun |
title |
Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury |
title_short |
Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury |
title_full |
Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury |
title_fullStr |
Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury |
title_full_unstemmed |
Study of Effect of Salvianolic Acid B on Motor Function Recovery in Rats with Spinal Cord Injury |
title_sort |
study of effect of salvianolic acid b on motor function recovery in rats with spinal cord injury |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2014-01-01 |
description |
In this study effect of salvianolic acid B was observed on motor function recovery of rats with spinal cord injury. 50 rats were selected and after inducing SCI their recovery under controlled conditions was studied using Sal B and PBS (as control). Both compounds were introduced intraperitoneally in respective groups of traumatic rats at the same time intervals for 28 days. It was observed that Sal B introduced at 5 mg/kg/day resulted in better motor function recovery. BBB score was recorded which increased significantly along with the reduction in cavity area observed by bright field microscopy of tissues, that is, from 1 to 10 and from 0.20±0.05 mm2 to 0.10±0.03 mm2, in Sal B treated group, respectively, compared to PBS group. Statistical analysis was carried out using SPSS software (SPSS, Chicago, IL, USA), values were expressed as mean ± SEM, and P value <0.01 was considered significant. Effect of Sal B on expression of NF-kB p65 and IkBα was studied and OD values of densitometry of western blots were taken. MPO activity was also studied. It was observed that treatment of Sal B significantly reduced the expression of both compounds in Sal B treated group as compared to control group after 28 days of treatment. |
url |
http://dx.doi.org/10.1155/2014/931850 |
work_keys_str_mv |
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