Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats
Background: Bleomycin-induced lung fibrosis has been accepted as an animal model for fibrosis in rats. The aim of this study was to evaluate the effects of saffron aqueous extract on this disorder paving the way for more investigation in treating idiopathic pulmonary fibrosis in human. Methods: M...
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doaj-62f6004189ba4893903100d3bfd0667b2020-11-24T22:30:50ZengArak University of Medical SciencesIranian Journal of Toxicology2008-29672251-94592017-11-01116712Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in RatsMehrzad Bahtouee 0Hossein Fatemikia 1Khalil Pourkhalili 2Euikyung Kim 3Hamid Reza Rahimi 4Zahra Tavosi 5 Abdol hamid.Esmaili 6Abdollah Hajivandi 7Yaghoob Hassan 8Ramin Seyedian9Department of Internal Medicine, Bushehr University of Medical Sciences, Bushehr, Iran. Department of Physiology, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran.Department of Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju, South Korea.Department of Pathology, Bushehr University of Medical Sciences, Bushehr, Iran.Department of Internal Medicine, Bushehr University of Medical Sciences, Bushehr, Iran. Department of Pathology, Bushehr University of Medical Sciences, Bushehr, Iran.Department of Biostatistics, Bushehr University of Medical Sciences, Bushehr,Iran.MSc of Nutrition, Research Center, Bushehr University of Medical Science, Bushehr, Iran.Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran. *Corresponding Author: E-Mail: raminseyedian@gmail.comBackground: Bleomycin-induced lung fibrosis has been accepted as an animal model for fibrosis in rats. The aim of this study was to evaluate the effects of saffron aqueous extract on this disorder paving the way for more investigation in treating idiopathic pulmonary fibrosis in human. Methods: Male Wistar rats (250–300 gr) were instilled a single dose of bleomycin (5 mg/kg) via intratracheal tube (n=6) in 2015. Sham group received normal saline. Saffron aqueous extract (50 mg/kg and 100 mg/kg) were given orally in two different treated groups with bleomycin for 28 days. Lung Indices was calculated at the end of this experiment. Lung segments fixed in 10% formaldehyde were used for pathological preparation with Hematoxylin & Eosin and trichrome staining. Results: The body weight was decreased and lung Indices increased in bleomycin group (P<0.5). Bleomycin administration increased myeloperoxidase, malondialdehyde and finally TNF-α in lung tissue homogenates (P<0.05) compared with sham group. The fibrotic process and thickening of alveolar septa in treated rats with bleomycin were increased by H&E and Masson Trichrome staining. Saffron treatment (50 and 100 mg/kg) attenuated the increase in MDA (264.43±10.4 nmol/g by the higher dose versus 378.4±18.1nmol/g), MPO (0.19±0.03 and 0.13± 0.04 IU/ml versus 0.39.2±0.05 IU/ml) and TNF-α level (18.42±3.7 ng/ml and14.31±3.6 ng /ml versus 35.32±4.2) in lung homogenates compared to bleomycin group (P<0.05). It decreased collagen accumulation and alveolar destructive patterns in pulmonary fibrosis. Conclusion: This study introduces saffron as novel anti-fibrotic agent against bleomycin-induced fibrosis due to histological examinations and preventive effects on destructive enzyme release in rats. https://ijt.arakmu.ac.ir/article-1-599-en.pdfBleomycinFibrosisRatSaffron |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mehrzad Bahtouee Hossein Fatemikia Khalil Pourkhalili Euikyung Kim Hamid Reza Rahimi Zahra Tavosi Abdol hamid.Esmaili Abdollah Hajivandi Yaghoob Hassan Ramin Seyedian |
spellingShingle |
Mehrzad Bahtouee Hossein Fatemikia Khalil Pourkhalili Euikyung Kim Hamid Reza Rahimi Zahra Tavosi Abdol hamid.Esmaili Abdollah Hajivandi Yaghoob Hassan Ramin Seyedian Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats Iranian Journal of Toxicology Bleomycin Fibrosis Rat Saffron |
author_facet |
Mehrzad Bahtouee Hossein Fatemikia Khalil Pourkhalili Euikyung Kim Hamid Reza Rahimi Zahra Tavosi Abdol hamid.Esmaili Abdollah Hajivandi Yaghoob Hassan Ramin Seyedian |
author_sort |
Mehrzad Bahtouee |
title |
Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats |
title_short |
Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats |
title_full |
Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats |
title_fullStr |
Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats |
title_full_unstemmed |
Saffron Protection against Bleomycin-Induced Pulmonary Fibrosis in Rats |
title_sort |
saffron protection against bleomycin-induced pulmonary fibrosis in rats |
publisher |
Arak University of Medical Sciences |
series |
Iranian Journal of Toxicology |
issn |
2008-2967 2251-9459 |
publishDate |
2017-11-01 |
description |
Background: Bleomycin-induced lung fibrosis has been accepted as an animal model for fibrosis in rats. The aim of this study was to evaluate the effects of saffron aqueous extract on this disorder paving the way for more investigation in treating idiopathic pulmonary fibrosis in human.
Methods: Male Wistar rats (250–300 gr) were instilled a single dose of bleomycin (5 mg/kg) via intratracheal tube (n=6) in 2015. Sham group received normal saline. Saffron aqueous extract (50 mg/kg and 100 mg/kg) were given orally in two different treated groups with bleomycin for 28 days. Lung Indices was calculated at the end of this experiment. Lung segments fixed in 10% formaldehyde were used for pathological preparation with Hematoxylin & Eosin and trichrome staining.
Results: The body weight was decreased and lung Indices increased in bleomycin group (P<0.5). Bleomycin administration increased myeloperoxidase, malondialdehyde and finally TNF-α in lung tissue homogenates (P<0.05) compared with sham group. The fibrotic process and thickening of alveolar septa in treated rats with bleomycin were increased by H&E and Masson Trichrome staining. Saffron treatment (50 and 100 mg/kg) attenuated the increase in MDA (264.43±10.4 nmol/g by the higher dose versus 378.4±18.1nmol/g), MPO (0.19±0.03 and 0.13± 0.04 IU/ml versus 0.39.2±0.05 IU/ml) and TNF-α level (18.42±3.7 ng/ml and14.31±3.6 ng /ml versus 35.32±4.2) in lung homogenates compared to bleomycin group (P<0.05). It decreased collagen accumulation and alveolar destructive patterns in pulmonary fibrosis.
Conclusion: This study introduces saffron as novel anti-fibrotic agent against bleomycin-induced fibrosis due to histological examinations and preventive effects on destructive enzyme release in rats.
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topic |
Bleomycin Fibrosis Rat Saffron |
url |
https://ijt.arakmu.ac.ir/article-1-599-en.pdf |
work_keys_str_mv |
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