Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy

Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here...

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Main Authors: Dari F. Da, Ruth McCabe, Bernard M. Somé, Pedro M. Esperança, Katarzyna A. Sala, Josua Blight, Andrew M. Blagborough, Floyd Dowell, Serge R. Yerbanga, Thierry Lefèvre, Karine Mouline, Roch K. Dabiré, Thomas S. Churcher
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-89715-1
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spelling doaj-62f6f2a286bc46f793a6a8c2cea546b12021-05-16T11:25:36ZengNature Publishing GroupScientific Reports2045-23222021-05-011111810.1038/s41598-021-89715-1Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopyDari F. Da0Ruth McCabe1Bernard M. Somé2Pedro M. Esperança3Katarzyna A. Sala4Josua Blight5Andrew M. Blagborough6Floyd Dowell7Serge R. Yerbanga8Thierry Lefèvre9Karine Mouline10Roch K. Dabiré11Thomas S. Churcher12Institut de Recherche en Sciences de la SantéMRC Centre for Global Infectious Disease Analysis, Infectious Disease Epidemiology, Imperial College LondonInstitut de Recherche en Sciences de la SantéMRC Centre for Global Infectious Disease Analysis, Infectious Disease Epidemiology, Imperial College LondonDivision of Microbiology and Parasitology, Department of Pathology, Cambridge UniversityDepartment of Life Sciences, Imperial College LondonDivision of Microbiology and Parasitology, Department of Pathology, Cambridge UniversityStored Product Insect and Engineering Research Unit, United States Department of Agriculture/Agricultural Research Services, Center for Grain and Animal Health ResearchInstitut de Recherche en Sciences de la SantéMIVEGEC, Montpellier University, IRD, CNRSMIVEGEC, Montpellier University, IRD, CNRSInstitut de Recherche en Sciences de la SantéMRC Centre for Global Infectious Disease Analysis, Infectious Disease Epidemiology, Imperial College LondonAbstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected field caught mosquitoes. Models trained on field mosquitoes were unable to predict the infection status of other field mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specificity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.https://doi.org/10.1038/s41598-021-89715-1
collection DOAJ
language English
format Article
sources DOAJ
author Dari F. Da
Ruth McCabe
Bernard M. Somé
Pedro M. Esperança
Katarzyna A. Sala
Josua Blight
Andrew M. Blagborough
Floyd Dowell
Serge R. Yerbanga
Thierry Lefèvre
Karine Mouline
Roch K. Dabiré
Thomas S. Churcher
spellingShingle Dari F. Da
Ruth McCabe
Bernard M. Somé
Pedro M. Esperança
Katarzyna A. Sala
Josua Blight
Andrew M. Blagborough
Floyd Dowell
Serge R. Yerbanga
Thierry Lefèvre
Karine Mouline
Roch K. Dabiré
Thomas S. Churcher
Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
Scientific Reports
author_facet Dari F. Da
Ruth McCabe
Bernard M. Somé
Pedro M. Esperança
Katarzyna A. Sala
Josua Blight
Andrew M. Blagborough
Floyd Dowell
Serge R. Yerbanga
Thierry Lefèvre
Karine Mouline
Roch K. Dabiré
Thomas S. Churcher
author_sort Dari F. Da
title Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_short Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_full Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_fullStr Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_full_unstemmed Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_sort detection of plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-05-01
description Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected field caught mosquitoes. Models trained on field mosquitoes were unable to predict the infection status of other field mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specificity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.
url https://doi.org/10.1038/s41598-021-89715-1
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