Effects of Hypergravity on Osteopontin Expression in Osteoblasts.
Mechanical stimuli play crucial roles in bone remodeling and resorption. Osteopontin (OPN), a marker for osteoblasts, is important in cell communication and matrix mineralization, and is known to function during mechanotransduction. Hypergravity is a convenient approach to forge mechanical stimuli o...
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2015-01-01
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doaj-630ea394ea6b45f88f4608e18bc212272020-11-24T21:24:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012884610.1371/journal.pone.0128846Effects of Hypergravity on Osteopontin Expression in Osteoblasts.Shuai ZhouYan ZuZhenglong SunFengyuan ZhuangChun YangMechanical stimuli play crucial roles in bone remodeling and resorption. Osteopontin (OPN), a marker for osteoblasts, is important in cell communication and matrix mineralization, and is known to function during mechanotransduction. Hypergravity is a convenient approach to forge mechanical stimuli on cells. It has positive effects on certain markers of osteoblast maturation, making it a possible strategy for bone tissue engineering. We investigated the effects of hypergravity on OPN expression and cell signaling in osteoblasts. Hypergravity treatment at 20 g for 24 hours upregulated OPN expression in MC3T3-E1 cells at the protein as well as mRNA level. Hypergravity promoted OPN expression by facilitating focal adhesion assembly, strengthening actin bundles, and increasing Runx2 expression. In the hypergravity-triggered OPN expression pathway, focal adhesion assembly-associated FAK phosphorylation was upstream of actin bundle assembly.http://europepmc.org/articles/PMC4457898?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuai Zhou Yan Zu Zhenglong Sun Fengyuan Zhuang Chun Yang |
spellingShingle |
Shuai Zhou Yan Zu Zhenglong Sun Fengyuan Zhuang Chun Yang Effects of Hypergravity on Osteopontin Expression in Osteoblasts. PLoS ONE |
author_facet |
Shuai Zhou Yan Zu Zhenglong Sun Fengyuan Zhuang Chun Yang |
author_sort |
Shuai Zhou |
title |
Effects of Hypergravity on Osteopontin Expression in Osteoblasts. |
title_short |
Effects of Hypergravity on Osteopontin Expression in Osteoblasts. |
title_full |
Effects of Hypergravity on Osteopontin Expression in Osteoblasts. |
title_fullStr |
Effects of Hypergravity on Osteopontin Expression in Osteoblasts. |
title_full_unstemmed |
Effects of Hypergravity on Osteopontin Expression in Osteoblasts. |
title_sort |
effects of hypergravity on osteopontin expression in osteoblasts. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
Mechanical stimuli play crucial roles in bone remodeling and resorption. Osteopontin (OPN), a marker for osteoblasts, is important in cell communication and matrix mineralization, and is known to function during mechanotransduction. Hypergravity is a convenient approach to forge mechanical stimuli on cells. It has positive effects on certain markers of osteoblast maturation, making it a possible strategy for bone tissue engineering. We investigated the effects of hypergravity on OPN expression and cell signaling in osteoblasts. Hypergravity treatment at 20 g for 24 hours upregulated OPN expression in MC3T3-E1 cells at the protein as well as mRNA level. Hypergravity promoted OPN expression by facilitating focal adhesion assembly, strengthening actin bundles, and increasing Runx2 expression. In the hypergravity-triggered OPN expression pathway, focal adhesion assembly-associated FAK phosphorylation was upstream of actin bundle assembly. |
url |
http://europepmc.org/articles/PMC4457898?pdf=render |
work_keys_str_mv |
AT shuaizhou effectsofhypergravityonosteopontinexpressioninosteoblasts AT yanzu effectsofhypergravityonosteopontinexpressioninosteoblasts AT zhenglongsun effectsofhypergravityonosteopontinexpressioninosteoblasts AT fengyuanzhuang effectsofhypergravityonosteopontinexpressioninosteoblasts AT chunyang effectsofhypergravityonosteopontinexpressioninosteoblasts |
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1725989170701139968 |