MALDI imaging MS of phospholipids in the mouse lung[S]
Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are PUFAs that are present in phospholipids in cell membranes. In this study, MALDI imaging MS was used to determine the localization of arachidonate- and d...
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doaj-6314e5e4164248c6be0cfeab847bacda2021-04-28T06:01:26ZengElsevierJournal of Lipid Research0022-22752011-08-0152815511560MALDI imaging MS of phospholipids in the mouse lung[S]Karin A. Zemski Berry0Bilan Li1Susan D. Reynolds2Robert M. Barkley3Miguel A. Gijón4Joseph A. Hankin5Peter M. Henson6Robert C. Murphy7Department of Pharmacology, University of Colorado Denver, 12801 East 17th Avenue, Mail Stop 8303, Aurora, CO 80045Department of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206Department of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206Department of Pharmacology, University of Colorado Denver, 12801 East 17th Avenue, Mail Stop 8303, Aurora, CO 80045Department of Pharmacology, University of Colorado Denver, 12801 East 17th Avenue, Mail Stop 8303, Aurora, CO 80045Department of Pharmacology, University of Colorado Denver, 12801 East 17th Avenue, Mail Stop 8303, Aurora, CO 80045Department of Pediatrics, National Jewish Health, 1400 Jackson Street, Denver, CO 80206To whom correspondence should be addressed.; Department of Pharmacology, University of Colorado Denver, 12801 East 17th Avenue, Mail Stop 8303, Aurora, CO 80045; To whom correspondence should be addressed.Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are PUFAs that are present in phospholipids in cell membranes. In this study, MALDI imaging MS was used to determine the localization of arachidonate- and docosahexaenoate-containing phospholipids in mouse lung. These PUFA-containing phospholipids were determined to be uniquely abundant at the lining of small and large airways, which were unequivocally identified by immunohistochemistry. In addition, it was found that the blood vessels present in the lung were characterized by sphingomyelin molecular species, and lung surfactant phospholipids appeared evenly distributed throughout the lung parenchyma, indicating alveolar localization. This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology.http://www.sciencedirect.com/science/article/pii/S0022227520369315arachidonic acidlipid localizationcryoembedding agentlipid mediatorspulmonary tissuemass spectrometry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karin A. Zemski Berry Bilan Li Susan D. Reynolds Robert M. Barkley Miguel A. Gijón Joseph A. Hankin Peter M. Henson Robert C. Murphy |
spellingShingle |
Karin A. Zemski Berry Bilan Li Susan D. Reynolds Robert M. Barkley Miguel A. Gijón Joseph A. Hankin Peter M. Henson Robert C. Murphy MALDI imaging MS of phospholipids in the mouse lung[S] Journal of Lipid Research arachidonic acid lipid localization cryoembedding agent lipid mediators pulmonary tissue mass spectrometry |
author_facet |
Karin A. Zemski Berry Bilan Li Susan D. Reynolds Robert M. Barkley Miguel A. Gijón Joseph A. Hankin Peter M. Henson Robert C. Murphy |
author_sort |
Karin A. Zemski Berry |
title |
MALDI imaging MS of phospholipids in the mouse lung[S] |
title_short |
MALDI imaging MS of phospholipids in the mouse lung[S] |
title_full |
MALDI imaging MS of phospholipids in the mouse lung[S] |
title_fullStr |
MALDI imaging MS of phospholipids in the mouse lung[S] |
title_full_unstemmed |
MALDI imaging MS of phospholipids in the mouse lung[S] |
title_sort |
maldi imaging ms of phospholipids in the mouse lung[s] |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2011-08-01 |
description |
Lipid mediators are important in lung biochemistry and are derived from the enzymatic oxidation of arachidonic and docosahexaenoic acids, which are PUFAs that are present in phospholipids in cell membranes. In this study, MALDI imaging MS was used to determine the localization of arachidonate- and docosahexaenoate-containing phospholipids in mouse lung. These PUFA-containing phospholipids were determined to be uniquely abundant at the lining of small and large airways, which were unequivocally identified by immunohistochemistry. In addition, it was found that the blood vessels present in the lung were characterized by sphingomyelin molecular species, and lung surfactant phospholipids appeared evenly distributed throughout the lung parenchyma, indicating alveolar localization. This technique revealed unexpected high concentrations of arachidonate- and docosahexaenoate-containing phospholipids lining the airways in pulmonary tissue, which could serve as precursors of lipid mediators affecting airways biology. |
topic |
arachidonic acid lipid localization cryoembedding agent lipid mediators pulmonary tissue mass spectrometry |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520369315 |
work_keys_str_mv |
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1721504484840964096 |