Chemerin deficiency regulates adipogenesis is depot different through TIMP1
Adipocytes and immune cells are vital for the development of adipose tissue. Adipokines secreted by adipocytes regulate adipogenesis and body metabolism. Chemerin is one of the adipokines. However, the function and mechanism of chemerin in adipose tissue are not fully illuminated. Compared with wild...
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doaj-634bb00ecc8d4a3b818a10d5a63809582021-07-15T04:27:53ZengElsevierGenes and Diseases2352-30422021-09-0185698708Chemerin deficiency regulates adipogenesis is depot different through TIMP1Cheng-Long Huang0Liu-Ling Xiao1Min Xu2Jun Li3Shu-Fen Li4Cui-Song Zhu5Yu-Li Lin6Rui He7Xi Li8Biology Science Institutes, Chongqing Medical University, Chongqing, 400016, PR ChinaCenter for Translational Research in Hematologic Malignancies, Houston Methodist Cancer Center, Houston Methodist Research Institute, Houston, TX 77030, USA; Key Laboratory of Metabolic Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai, 200032, PR ChinaBiology Science Institutes, Chongqing Medical University, Chongqing, 400016, PR ChinaBiology Science Institutes, Chongqing Medical University, Chongqing, 400016, PR ChinaKey Laboratory of Metabolic Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai, 200032, PR ChinaKey Laboratory of Metabolic Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai, 200032, PR China; Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, PR ChinaDepartment of Immunology, Fudan University Shanghai Medical College, Shanghai, 200032, PR ChinaDepartment of Immunology, Fudan University Shanghai Medical College, Shanghai, 200032, PR ChinaBiology Science Institutes, Chongqing Medical University, Chongqing, 400016, PR China; Corresponding author. Biology Science Institutes, Chongqing Medical University, 1 Yi Xue Yuan Road, Chongqing 400032, PR China.Adipocytes and immune cells are vital for the development of adipose tissue. Adipokines secreted by adipocytes regulate adipogenesis and body metabolism. Chemerin is one of the adipokines. However, the function and mechanism of chemerin in adipose tissue are not fully illuminated. Compared with wild type (WT) mice, Rarres2−/− mice gained weight and significantly increased fat distribution in subcutaneous adipose tissue (SAT), rather than visceral adipose tissue (VAT) on high fat diet (HFD). PPARγ and C/EBPα, the master regulators of adipogenesis, were up-regulated in SAT and down-regulated in VAT in Rarres2−/− mice comparing with WT mice. Inspite of chemerin deficiency or not, the ratio of adipocyte-progenitors to total cells and the differentiation capacity of adipocyte-progenitors were similar in SAT and VAT, but macrophage infiltration in VAT was more severe than in SAT in Rarres2−/− mice. Furthermore, CD45+ immune cells supernatant from Rarres2−/− SAT promoted the differentiation of adipocyte-progenitors and 3T3-L1 cells. Adipokine array assay of CD45+ immune cells supernatant revealed that metalloproteinase inhibitor 1 (TIMP1), an inhibitor of adipogenesis, was reduced in Rarres2−/− SAT, but increased in Rarres2−/− VAT. As we specifically knocked down chemerin in SAT, TIMP1 was down-regulated and adipogenesis was promoted with reducing infiltration of macrophages. The present study demonstrates that the effects of chemerin on adipose tissue is depot different, and specific knock down chemerin in SAT promote adipogenesis and improve glucose tolerance test (GTT) and insulin tolerance test (ITT). This suggests a potential therapeutic target for chemerin in the treatment of obesity related metabolic disorder.http://www.sciencedirect.com/science/article/pii/S2352304220300556AdipogenesisAdipose tissue depotChemerinInflammationTIMP1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cheng-Long Huang Liu-Ling Xiao Min Xu Jun Li Shu-Fen Li Cui-Song Zhu Yu-Li Lin Rui He Xi Li |
spellingShingle |
Cheng-Long Huang Liu-Ling Xiao Min Xu Jun Li Shu-Fen Li Cui-Song Zhu Yu-Li Lin Rui He Xi Li Chemerin deficiency regulates adipogenesis is depot different through TIMP1 Genes and Diseases Adipogenesis Adipose tissue depot Chemerin Inflammation TIMP1 |
author_facet |
Cheng-Long Huang Liu-Ling Xiao Min Xu Jun Li Shu-Fen Li Cui-Song Zhu Yu-Li Lin Rui He Xi Li |
author_sort |
Cheng-Long Huang |
title |
Chemerin deficiency regulates adipogenesis is depot different through TIMP1 |
title_short |
Chemerin deficiency regulates adipogenesis is depot different through TIMP1 |
title_full |
Chemerin deficiency regulates adipogenesis is depot different through TIMP1 |
title_fullStr |
Chemerin deficiency regulates adipogenesis is depot different through TIMP1 |
title_full_unstemmed |
Chemerin deficiency regulates adipogenesis is depot different through TIMP1 |
title_sort |
chemerin deficiency regulates adipogenesis is depot different through timp1 |
publisher |
Elsevier |
series |
Genes and Diseases |
issn |
2352-3042 |
publishDate |
2021-09-01 |
description |
Adipocytes and immune cells are vital for the development of adipose tissue. Adipokines secreted by adipocytes regulate adipogenesis and body metabolism. Chemerin is one of the adipokines. However, the function and mechanism of chemerin in adipose tissue are not fully illuminated. Compared with wild type (WT) mice, Rarres2−/− mice gained weight and significantly increased fat distribution in subcutaneous adipose tissue (SAT), rather than visceral adipose tissue (VAT) on high fat diet (HFD). PPARγ and C/EBPα, the master regulators of adipogenesis, were up-regulated in SAT and down-regulated in VAT in Rarres2−/− mice comparing with WT mice. Inspite of chemerin deficiency or not, the ratio of adipocyte-progenitors to total cells and the differentiation capacity of adipocyte-progenitors were similar in SAT and VAT, but macrophage infiltration in VAT was more severe than in SAT in Rarres2−/− mice. Furthermore, CD45+ immune cells supernatant from Rarres2−/− SAT promoted the differentiation of adipocyte-progenitors and 3T3-L1 cells. Adipokine array assay of CD45+ immune cells supernatant revealed that metalloproteinase inhibitor 1 (TIMP1), an inhibitor of adipogenesis, was reduced in Rarres2−/− SAT, but increased in Rarres2−/− VAT. As we specifically knocked down chemerin in SAT, TIMP1 was down-regulated and adipogenesis was promoted with reducing infiltration of macrophages. The present study demonstrates that the effects of chemerin on adipose tissue is depot different, and specific knock down chemerin in SAT promote adipogenesis and improve glucose tolerance test (GTT) and insulin tolerance test (ITT). This suggests a potential therapeutic target for chemerin in the treatment of obesity related metabolic disorder. |
topic |
Adipogenesis Adipose tissue depot Chemerin Inflammation TIMP1 |
url |
http://www.sciencedirect.com/science/article/pii/S2352304220300556 |
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