Characterization of human decidual mast cells and establishment of a culture system
Background: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human deci...
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doaj-637b9344509a45938e0908c160ba275a2020-11-25T01:43:57ZengElsevierAllergology International1323-89302018-09-0167S18S24Characterization of human decidual mast cells and establishment of a culture systemTakayuki Matsuno0Shota Toyoshima1Tomomi Sakamoto-Sasaki2Jun-ichi Kashiwakura3Akira Matsuda4Yasuo Watanabe5Hiromitsu Azuma6Kei Kawana7Tatsuo Yamamoto8Yoshimichi Okayama9Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan; Allergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, JapanAllergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, Japan; Center for Institutional Research and Medical Education, Nihon University School of Medicine, Tokyo, JapanAllergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, Japan; Center for Institutional Research and Medical Education, Nihon University School of Medicine, Tokyo, JapanDepartment of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, JapanDepartment of Ophthalmology, Juntendo University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Juntendo University School of Medicine, Tokyo, JapanDepartment of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, JapanDepartment of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, JapanDepartment of Obstetrics and Gynecology, Kasukabe Medical Center, Saitama, JapanAllergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, Japan; Center for Institutional Research and Medical Education, Nihon University School of Medicine, Tokyo, Japan; Corresponding author. Allergy and Immunology Project Team, Center for Institutional Research and Medical Education, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, Japan.Background: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human decidua and to establish a culture system for decidua-derived MCs. Methods: Decidual tissues were obtained from patients who underwent a legal elective abortion (6th week to 9th week of pregnancy), and decidual MCs were enzymatically dispersed. Cultured decidua-derived MCs were generated by culturing decidual cells with stem cell factor. An ultrastructural analysis of primary decidual MCs and cultured decidua-derived MCs was performed using a transmission electron microscope. Receptor and protease expression was analyzed using FACS. Histamine released from MCs was measured using enzyme immune assays. Results: A larger proportion of tryptase positive(+) MCs in decidua was present on the maternal side. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs showed an FcεRIα+Kit+tryptase+chymase+ phenotype. Their granules contenting particles exhibited variable amounts of electron-lucent space separating electron-dense particles. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs released comparable amounts of histamine following FcεRI aggregation. Conclusions: The isolation method for MCs from decidua during early pregnancy and the culture system for decidua-derived MCs may enable the roles of decidual MC during pregnancy to be explored. Keywords: Cultured decidua-derived mast cells, Decidua, FcεRI, Mast cell, Pregnancyhttp://www.sciencedirect.com/science/article/pii/S1323893018300558 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takayuki Matsuno Shota Toyoshima Tomomi Sakamoto-Sasaki Jun-ichi Kashiwakura Akira Matsuda Yasuo Watanabe Hiromitsu Azuma Kei Kawana Tatsuo Yamamoto Yoshimichi Okayama |
spellingShingle |
Takayuki Matsuno Shota Toyoshima Tomomi Sakamoto-Sasaki Jun-ichi Kashiwakura Akira Matsuda Yasuo Watanabe Hiromitsu Azuma Kei Kawana Tatsuo Yamamoto Yoshimichi Okayama Characterization of human decidual mast cells and establishment of a culture system Allergology International |
author_facet |
Takayuki Matsuno Shota Toyoshima Tomomi Sakamoto-Sasaki Jun-ichi Kashiwakura Akira Matsuda Yasuo Watanabe Hiromitsu Azuma Kei Kawana Tatsuo Yamamoto Yoshimichi Okayama |
author_sort |
Takayuki Matsuno |
title |
Characterization of human decidual mast cells and establishment of a culture system |
title_short |
Characterization of human decidual mast cells and establishment of a culture system |
title_full |
Characterization of human decidual mast cells and establishment of a culture system |
title_fullStr |
Characterization of human decidual mast cells and establishment of a culture system |
title_full_unstemmed |
Characterization of human decidual mast cells and establishment of a culture system |
title_sort |
characterization of human decidual mast cells and establishment of a culture system |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2018-09-01 |
description |
Background: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human decidua and to establish a culture system for decidua-derived MCs. Methods: Decidual tissues were obtained from patients who underwent a legal elective abortion (6th week to 9th week of pregnancy), and decidual MCs were enzymatically dispersed. Cultured decidua-derived MCs were generated by culturing decidual cells with stem cell factor. An ultrastructural analysis of primary decidual MCs and cultured decidua-derived MCs was performed using a transmission electron microscope. Receptor and protease expression was analyzed using FACS. Histamine released from MCs was measured using enzyme immune assays. Results: A larger proportion of tryptase positive(+) MCs in decidua was present on the maternal side. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs showed an FcεRIα+Kit+tryptase+chymase+ phenotype. Their granules contenting particles exhibited variable amounts of electron-lucent space separating electron-dense particles. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs released comparable amounts of histamine following FcεRI aggregation. Conclusions: The isolation method for MCs from decidua during early pregnancy and the culture system for decidua-derived MCs may enable the roles of decidual MC during pregnancy to be explored. Keywords: Cultured decidua-derived mast cells, Decidua, FcεRI, Mast cell, Pregnancy |
url |
http://www.sciencedirect.com/science/article/pii/S1323893018300558 |
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