Characterization of human decidual mast cells and establishment of a culture system

Background: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human deci...

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Main Authors: Takayuki Matsuno, Shota Toyoshima, Tomomi Sakamoto-Sasaki, Jun-ichi Kashiwakura, Akira Matsuda, Yasuo Watanabe, Hiromitsu Azuma, Kei Kawana, Tatsuo Yamamoto, Yoshimichi Okayama
Format: Article
Language:English
Published: Elsevier 2018-09-01
Series:Allergology International
Online Access:http://www.sciencedirect.com/science/article/pii/S1323893018300558
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spelling doaj-637b9344509a45938e0908c160ba275a2020-11-25T01:43:57ZengElsevierAllergology International1323-89302018-09-0167S18S24Characterization of human decidual mast cells and establishment of a culture systemTakayuki Matsuno0Shota Toyoshima1Tomomi Sakamoto-Sasaki2Jun-ichi Kashiwakura3Akira Matsuda4Yasuo Watanabe5Hiromitsu Azuma6Kei Kawana7Tatsuo Yamamoto8Yoshimichi Okayama9Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan; Allergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, JapanAllergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, Japan; Center for Institutional Research and Medical Education, Nihon University School of Medicine, Tokyo, JapanAllergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, Japan; Center for Institutional Research and Medical Education, Nihon University School of Medicine, Tokyo, JapanDepartment of Immunology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, JapanDepartment of Ophthalmology, Juntendo University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Juntendo University School of Medicine, Tokyo, JapanDepartment of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, JapanDepartment of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, JapanDepartment of Obstetrics and Gynecology, Kasukabe Medical Center, Saitama, JapanAllergy and Immunology Project Team, Nihon University School of Medicine, Tokyo, Japan; Center for Institutional Research and Medical Education, Nihon University School of Medicine, Tokyo, Japan; Corresponding author. Allergy and Immunology Project Team, Center for Institutional Research and Medical Education, Nihon University School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, Japan.Background: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human decidua and to establish a culture system for decidua-derived MCs. Methods: Decidual tissues were obtained from patients who underwent a legal elective abortion (6th week to 9th week of pregnancy), and decidual MCs were enzymatically dispersed. Cultured decidua-derived MCs were generated by culturing decidual cells with stem cell factor. An ultrastructural analysis of primary decidual MCs and cultured decidua-derived MCs was performed using a transmission electron microscope. Receptor and protease expression was analyzed using FACS. Histamine released from MCs was measured using enzyme immune assays. Results: A larger proportion of tryptase positive(+) MCs in decidua was present on the maternal side. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs showed an FcεRIα+Kit+tryptase+chymase+ phenotype. Their granules contenting particles exhibited variable amounts of electron-lucent space separating electron-dense particles. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs released comparable amounts of histamine following FcεRI aggregation. Conclusions: The isolation method for MCs from decidua during early pregnancy and the culture system for decidua-derived MCs may enable the roles of decidual MC during pregnancy to be explored. Keywords: Cultured decidua-derived mast cells, Decidua, FcεRI, Mast cell, Pregnancyhttp://www.sciencedirect.com/science/article/pii/S1323893018300558
collection DOAJ
language English
format Article
sources DOAJ
author Takayuki Matsuno
Shota Toyoshima
Tomomi Sakamoto-Sasaki
Jun-ichi Kashiwakura
Akira Matsuda
Yasuo Watanabe
Hiromitsu Azuma
Kei Kawana
Tatsuo Yamamoto
Yoshimichi Okayama
spellingShingle Takayuki Matsuno
Shota Toyoshima
Tomomi Sakamoto-Sasaki
Jun-ichi Kashiwakura
Akira Matsuda
Yasuo Watanabe
Hiromitsu Azuma
Kei Kawana
Tatsuo Yamamoto
Yoshimichi Okayama
Characterization of human decidual mast cells and establishment of a culture system
Allergology International
author_facet Takayuki Matsuno
Shota Toyoshima
Tomomi Sakamoto-Sasaki
Jun-ichi Kashiwakura
Akira Matsuda
Yasuo Watanabe
Hiromitsu Azuma
Kei Kawana
Tatsuo Yamamoto
Yoshimichi Okayama
author_sort Takayuki Matsuno
title Characterization of human decidual mast cells and establishment of a culture system
title_short Characterization of human decidual mast cells and establishment of a culture system
title_full Characterization of human decidual mast cells and establishment of a culture system
title_fullStr Characterization of human decidual mast cells and establishment of a culture system
title_full_unstemmed Characterization of human decidual mast cells and establishment of a culture system
title_sort characterization of human decidual mast cells and establishment of a culture system
publisher Elsevier
series Allergology International
issn 1323-8930
publishDate 2018-09-01
description Background: Although rodent decidual mast cells (MCs) reportedly play an important role in implantation and placenta formation, the characterization of human decidual MCs has been not well clarified. The aims of this study were to investigate the distribution and characteristics of MCs in human decidua and to establish a culture system for decidua-derived MCs. Methods: Decidual tissues were obtained from patients who underwent a legal elective abortion (6th week to 9th week of pregnancy), and decidual MCs were enzymatically dispersed. Cultured decidua-derived MCs were generated by culturing decidual cells with stem cell factor. An ultrastructural analysis of primary decidual MCs and cultured decidua-derived MCs was performed using a transmission electron microscope. Receptor and protease expression was analyzed using FACS. Histamine released from MCs was measured using enzyme immune assays. Results: A larger proportion of tryptase positive(+) MCs in decidua was present on the maternal side. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs showed an FcεRIα+Kit+tryptase+chymase+ phenotype. Their granules contenting particles exhibited variable amounts of electron-lucent space separating electron-dense particles. Both enzymatically dispersed decidual MCs and cultured decidua-derived MCs released comparable amounts of histamine following FcεRI aggregation. Conclusions: The isolation method for MCs from decidua during early pregnancy and the culture system for decidua-derived MCs may enable the roles of decidual MC during pregnancy to be explored. Keywords: Cultured decidua-derived mast cells, Decidua, FcεRI, Mast cell, Pregnancy
url http://www.sciencedirect.com/science/article/pii/S1323893018300558
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