In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish
Nitroxyl (HNO) plays a critical role in many physiological processes which includes vasorelaxation in heart failure, neuroregulation, and myocardial contractility. Powerful imaging tools are required to obtain information for understanding the mechanisms involved in these in vivo processes. In order...
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doaj-63801500fe5c47c8b1d010202472c65a2020-11-24T22:08:52ZengMDPI AGMolecules1420-30492018-10-012310255110.3390/molecules23102551molecules23102551In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and ZebrafishSathyadevi Palanisamy0Yu-Liang Wang1Yu-Jen Chen2Chiao-Yun Chen3Fu-Te Tsai4Wen-Feng Liaw5Yun-Ming Wang6Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center For Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, TaiwanDepartment of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center For Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, TaiwanDepartment of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center For Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, TaiwanDepartment of Radiology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Chemistry, National Tsing Hua University, Hsinchu 30043, TaiwanDepartment of Chemistry, National Tsing Hua University, Hsinchu 30043, TaiwanDepartment of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center For Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Chiao Tung University, 75 Bo-Ai Street, Hsinchu 300, TaiwanNitroxyl (HNO) plays a critical role in many physiological processes which includes vasorelaxation in heart failure, neuroregulation, and myocardial contractility. Powerful imaging tools are required to obtain information for understanding the mechanisms involved in these in vivo processes. In order to develop a rapid and high sensitive probe for HNO detection in living cells and the zebrafish model organism, 2-((2-(benzothiazole-2yl)benzylidene) amino)benzoic acid (AbTCA) as a ligand, and its corresponding copper(II) complex Cu(II)-AbTCA were synthesized. The reaction results of Cu(II)-AbTCA with Angeli’s salt showed that Cu(II)-AbTCA could detect HNO quantitatively in a range of 40–360 µM with a detection limit of 9.05 µM. Furthermore, Cu(II)-AbTCA is more selective towards HNO over other biological species including thiols, reactive nitrogen, and reactive oxygen species. Importantly, Cu(II)-AbTCA was successfully applied to detect HNO in living cells and zebrafish. The collective data reveals that Cu(II)-AbTCA could be used as a potential probe for HNO detection in living systems.http://www.mdpi.com/1420-3049/23/10/2551nitroxylfluorescent probeliving systemzebrafish (Danio rerio) |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sathyadevi Palanisamy Yu-Liang Wang Yu-Jen Chen Chiao-Yun Chen Fu-Te Tsai Wen-Feng Liaw Yun-Ming Wang |
spellingShingle |
Sathyadevi Palanisamy Yu-Liang Wang Yu-Jen Chen Chiao-Yun Chen Fu-Te Tsai Wen-Feng Liaw Yun-Ming Wang In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish Molecules nitroxyl fluorescent probe living system zebrafish (Danio rerio) |
author_facet |
Sathyadevi Palanisamy Yu-Liang Wang Yu-Jen Chen Chiao-Yun Chen Fu-Te Tsai Wen-Feng Liaw Yun-Ming Wang |
author_sort |
Sathyadevi Palanisamy |
title |
In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish |
title_short |
In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish |
title_full |
In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish |
title_fullStr |
In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish |
title_full_unstemmed |
In Vitro and in Vivo Imaging of Nitroxyl with Copper Fluorescent Probe in Living Cells and Zebrafish |
title_sort |
in vitro and in vivo imaging of nitroxyl with copper fluorescent probe in living cells and zebrafish |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-10-01 |
description |
Nitroxyl (HNO) plays a critical role in many physiological processes which includes vasorelaxation in heart failure, neuroregulation, and myocardial contractility. Powerful imaging tools are required to obtain information for understanding the mechanisms involved in these in vivo processes. In order to develop a rapid and high sensitive probe for HNO detection in living cells and the zebrafish model organism, 2-((2-(benzothiazole-2yl)benzylidene) amino)benzoic acid (AbTCA) as a ligand, and its corresponding copper(II) complex Cu(II)-AbTCA were synthesized. The reaction results of Cu(II)-AbTCA with Angeli’s salt showed that Cu(II)-AbTCA could detect HNO quantitatively in a range of 40–360 µM with a detection limit of 9.05 µM. Furthermore, Cu(II)-AbTCA is more selective towards HNO over other biological species including thiols, reactive nitrogen, and reactive oxygen species. Importantly, Cu(II)-AbTCA was successfully applied to detect HNO in living cells and zebrafish. The collective data reveals that Cu(II)-AbTCA could be used as a potential probe for HNO detection in living systems. |
topic |
nitroxyl fluorescent probe living system zebrafish (Danio rerio) |
url |
http://www.mdpi.com/1420-3049/23/10/2551 |
work_keys_str_mv |
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