A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion
Summary: Metastasis is the leading cause of death for patients with cancer. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumor growth toward malignancy. Advances in genome characterization technologies have been very success...
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doaj-63857e0e94984fed9ef235e03070465b2020-11-25T03:41:25ZengElsevieriScience2589-00422020-06-01236101237A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell InvasionBrenda Canales Coutiño0Zoe E. Cornhill1Africa Couto2Natalie A. Mack3Alexandra D. Rusu4Usha Nagarajan5Yuen Ngan Fan6Marina R. Hadjicharalambous7Marcos Castellanos Uribe8Amy Burrows9Anbarasu Lourdusamy10Ruman Rahman11Sean T. May12Marios Georgiou13School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; School of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Department of Biochemistry, School of Interdisciplinary and Applied Sciences, Central University of Haryana, Jant-Pali, Mahendergarh, Haryana, 123029, IndiaSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Faculty of Biology, Medicine & Health, University of Manchester, Manchester M13 9PL, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, UKSchool of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UKSchool of Medicine, University of Nottingham, Nottingham NG7 2UH, UKSchool of Medicine, University of Nottingham, Nottingham NG7 2UH, UKSchool of Biosciences, University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UKSchool of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Corresponding authorSummary: Metastasis is the leading cause of death for patients with cancer. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumor growth toward malignancy. Advances in genome characterization technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. However, the difficulty in evaluating whether these candidates drive tumor progression remains a major challenge. Using the genetic amenability of Drosophila melanogaster we generated tumors with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify conserved genes that enhance or suppress epithelial tumor progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved invasion suppressors. This includes constituents of the cohesin complex, whose loss of function either promotes individual or collective cell invasion, depending on the severity of effect on cohesin complex function.http://www.sciencedirect.com/science/article/pii/S2589004220304223Biological SciencesMolecular BiologyCell BiologyCancer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Brenda Canales Coutiño Zoe E. Cornhill Africa Couto Natalie A. Mack Alexandra D. Rusu Usha Nagarajan Yuen Ngan Fan Marina R. Hadjicharalambous Marcos Castellanos Uribe Amy Burrows Anbarasu Lourdusamy Ruman Rahman Sean T. May Marios Georgiou |
spellingShingle |
Brenda Canales Coutiño Zoe E. Cornhill Africa Couto Natalie A. Mack Alexandra D. Rusu Usha Nagarajan Yuen Ngan Fan Marina R. Hadjicharalambous Marcos Castellanos Uribe Amy Burrows Anbarasu Lourdusamy Ruman Rahman Sean T. May Marios Georgiou A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion iScience Biological Sciences Molecular Biology Cell Biology Cancer |
author_facet |
Brenda Canales Coutiño Zoe E. Cornhill Africa Couto Natalie A. Mack Alexandra D. Rusu Usha Nagarajan Yuen Ngan Fan Marina R. Hadjicharalambous Marcos Castellanos Uribe Amy Burrows Anbarasu Lourdusamy Ruman Rahman Sean T. May Marios Georgiou |
author_sort |
Brenda Canales Coutiño |
title |
A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion |
title_short |
A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion |
title_full |
A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion |
title_fullStr |
A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion |
title_full_unstemmed |
A Genetic Analysis of Tumor Progression in Drosophila Identifies the Cohesin Complex as a Suppressor of Individual and Collective Cell Invasion |
title_sort |
genetic analysis of tumor progression in drosophila identifies the cohesin complex as a suppressor of individual and collective cell invasion |
publisher |
Elsevier |
series |
iScience |
issn |
2589-0042 |
publishDate |
2020-06-01 |
description |
Summary: Metastasis is the leading cause of death for patients with cancer. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumor growth toward malignancy. Advances in genome characterization technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. However, the difficulty in evaluating whether these candidates drive tumor progression remains a major challenge. Using the genetic amenability of Drosophila melanogaster we generated tumors with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify conserved genes that enhance or suppress epithelial tumor progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved invasion suppressors. This includes constituents of the cohesin complex, whose loss of function either promotes individual or collective cell invasion, depending on the severity of effect on cohesin complex function. |
topic |
Biological Sciences Molecular Biology Cell Biology Cancer |
url |
http://www.sciencedirect.com/science/article/pii/S2589004220304223 |
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