A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma
Hai-wei Liang,* Zhi-hua Ye,* Shu-ya Yin, Wei-jia Mo, Han-lin Wang, Jin-che Zhao, Guo-mei Liang, Zhen-bo Feng, Gang Chen, Dian-zhong Luo Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China *These authors contributed equa...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Dove Medical Press
2017-07-01
|
Series: | OncoTargets and Therapy |
Subjects: | |
Online Access: | https://www.dovepress.com/a-comprehensive-insight-into-the-clinicopathologic-significance-of-mir-peer-reviewed-article-OTT |
id |
doaj-63a371b5f12b41478a519c0375296ec6 |
---|---|
record_format |
Article |
spelling |
doaj-63a371b5f12b41478a519c0375296ec62020-11-24T21:16:10ZengDove Medical PressOncoTargets and Therapy1178-69302017-07-01Volume 103405341933701A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinomaLiang HWYe ZHYin SYMo WJWang HLZhao JCLiang GMFeng ZBChen GLuo DZHai-wei Liang,* Zhi-hua Ye,* Shu-ya Yin, Wei-jia Mo, Han-lin Wang, Jin-che Zhao, Guo-mei Liang, Zhen-bo Feng, Gang Chen, Dian-zhong Luo Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China *These authors contributed equally to this work Background: Studies which focused on the character of miR-144-3p in hepatocellular carcinoma (HCC) are limited. This study aimed to explore the expression, clinical significance and the potential targets of miR-144-3p in HCC. Methods: The Cancer Genome Atlas (TCGA) and a cohort of 95 cases of HCC were applied to investigate aberrant miR-144-3p expression in HCC. A meta-analysis was performed to accumulate data on miR-144-3p expression in HCC based on TCGA, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Gene Expression Omnibus (GEO). Additionally, the potential regulatory mechanisms of miR-144-3p in HCC were explored by bioinformatics. Results: MiR-144-3p expression was downregulated distinctly in HCC compared to para-HCC tissue both in TCGA data (8.9139±1.5986 vs 10.7721±0.9156, P<0.001) and in our qRT-PCR validation (1.3208±0.7594 vs 2.6200±0.9263, P<0.001). The meta-analysis based on TCGA, qRT-PCR and GEO data confirmed a consistent result (standard mean difference =-0.854, 95% CI: -1.224 to -0.484, P<0.001). The receiver operating characteristic curve of miR-144-3p gained a significant diagnostic value both in TCGA data (area under the curve [AUC] =0.852, 95% CI: 0.810 to 0.894, P<0.001) and in qRT-PCR validation (AUC =0.867, 95% CI: 0.817 to 0.916, P<0.001), especially in alpha-fetoprotein–negative HCC patients (AUC =0.900, 95% CI: 0.839 to 0.960, P<0.001). Furthermore, we identified 119 potential targets of miR-144-3p in HCC by bioinformatics. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that several significant biologic functions and pathways correlated with the pathogenesis of HCC, including the p53 signaling pathway. Conclusion: MiR-144-3p may function as a cancer suppressor microRNA, which is essential for HCC progression through the regulation of various signaling pathways. Thus, interactions with miR-144-3p may provide a novel treatment strategy for HCC in the future. Keywords: miR-144-3p, hepatocellular carcinoma, TCGA, qRT-PCR, GEO, gene functional enrichment analysishttps://www.dovepress.com/a-comprehensive-insight-into-the-clinicopathologic-significance-of-mir-peer-reviewed-article-OTTmiR-144-3phepatocellular carcinomaTCGAqRT-PCRGEOgene functional enrichment analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Liang HW Ye ZH Yin SY Mo WJ Wang HL Zhao JC Liang GM Feng ZB Chen G Luo DZ |
spellingShingle |
Liang HW Ye ZH Yin SY Mo WJ Wang HL Zhao JC Liang GM Feng ZB Chen G Luo DZ A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma OncoTargets and Therapy miR-144-3p hepatocellular carcinoma TCGA qRT-PCR GEO gene functional enrichment analysis |
author_facet |
Liang HW Ye ZH Yin SY Mo WJ Wang HL Zhao JC Liang GM Feng ZB Chen G Luo DZ |
author_sort |
Liang HW |
title |
A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma |
title_short |
A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma |
title_full |
A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma |
title_fullStr |
A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma |
title_full_unstemmed |
A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma |
title_sort |
comprehensive insight into the clinicopathologic significance of mir-144-3p in hepatocellular carcinoma |
publisher |
Dove Medical Press |
series |
OncoTargets and Therapy |
issn |
1178-6930 |
publishDate |
2017-07-01 |
description |
Hai-wei Liang,* Zhi-hua Ye,* Shu-ya Yin, Wei-jia Mo, Han-lin Wang, Jin-che Zhao, Guo-mei Liang, Zhen-bo Feng, Gang Chen, Dian-zhong Luo Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, China *These authors contributed equally to this work Background: Studies which focused on the character of miR-144-3p in hepatocellular carcinoma (HCC) are limited. This study aimed to explore the expression, clinical significance and the potential targets of miR-144-3p in HCC. Methods: The Cancer Genome Atlas (TCGA) and a cohort of 95 cases of HCC were applied to investigate aberrant miR-144-3p expression in HCC. A meta-analysis was performed to accumulate data on miR-144-3p expression in HCC based on TCGA, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Gene Expression Omnibus (GEO). Additionally, the potential regulatory mechanisms of miR-144-3p in HCC were explored by bioinformatics. Results: MiR-144-3p expression was downregulated distinctly in HCC compared to para-HCC tissue both in TCGA data (8.9139±1.5986 vs 10.7721±0.9156, P<0.001) and in our qRT-PCR validation (1.3208±0.7594 vs 2.6200±0.9263, P<0.001). The meta-analysis based on TCGA, qRT-PCR and GEO data confirmed a consistent result (standard mean difference =-0.854, 95% CI: -1.224 to -0.484, P<0.001). The receiver operating characteristic curve of miR-144-3p gained a significant diagnostic value both in TCGA data (area under the curve [AUC] =0.852, 95% CI: 0.810 to 0.894, P<0.001) and in qRT-PCR validation (AUC =0.867, 95% CI: 0.817 to 0.916, P<0.001), especially in alpha-fetoprotein–negative HCC patients (AUC =0.900, 95% CI: 0.839 to 0.960, P<0.001). Furthermore, we identified 119 potential targets of miR-144-3p in HCC by bioinformatics. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that several significant biologic functions and pathways correlated with the pathogenesis of HCC, including the p53 signaling pathway. Conclusion: MiR-144-3p may function as a cancer suppressor microRNA, which is essential for HCC progression through the regulation of various signaling pathways. Thus, interactions with miR-144-3p may provide a novel treatment strategy for HCC in the future. Keywords: miR-144-3p, hepatocellular carcinoma, TCGA, qRT-PCR, GEO, gene functional enrichment analysis |
topic |
miR-144-3p hepatocellular carcinoma TCGA qRT-PCR GEO gene functional enrichment analysis |
url |
https://www.dovepress.com/a-comprehensive-insight-into-the-clinicopathologic-significance-of-mir-peer-reviewed-article-OTT |
work_keys_str_mv |
AT lianghw acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT yezh acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT yinsy acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT mowj acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT wanghl acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT zhaojc acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT lianggm acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT fengzb acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT cheng acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT luodz acomprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT lianghw comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT yezh comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT yinsy comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT mowj comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT wanghl comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT zhaojc comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT lianggm comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT fengzb comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT cheng comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma AT luodz comprehensiveinsightintotheclinicopathologicsignificanceofmir1443pinhepatocellularcarcinoma |
_version_ |
1726016734048026624 |