Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva
Andrographis paniculata leaf aqueous extract over 0.5, 1, 3, 5, and 24 h were determined to contain the highest amount of total phenolic compound and was used to evaluate mosquito larvicidal properties against Culex quinquefasciatus at varies concentrations (3.125, 6.25, 12.5, and 25 ppm) by determi...
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doaj-63af13daef1b4928b006572a180a388e2020-11-25T01:37:42ZengWalailak UniversityWalailak Journal of Science and Technology1686-39332228-835X2016-02-01132133140Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus LarvaWannee JIRAUNGKOORSKUL 0Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400 Thailand Andrographis paniculata leaf aqueous extract over 0.5, 1, 3, 5, and 24 h were determined to contain the highest amount of total phenolic compound and was used to evaluate mosquito larvicidal properties against Culex quinquefasciatus at varies concentrations (3.125, 6.25, 12.5, and 25 ppm) by determining the median and 90 % lethal concentration, LC50 and LC90, respectively, within 24 h and by histological analysis. The results revealed that the total phenolic compound measurement in each time extraction were 7.97, 8.68, 7.16, 6.70, and 5.78 mg/g GAE, respectively. The one hour aqueous extract of A. paniculata leaf expressed the 24-h LC50 and LC90 values in C. quinquefasciatus which were 15.93 and 28.23 ppm, respectively. Dose dependent lesions were observed. Under histological analysis, midgut lesions, i.e., separation of the epithelial cells from the basement membrane, elongation protruding into the lumen, disruption of the brush border, and the appearance of several vesicles and cytoplasm masses were observed in this study. The present study reveals that the aqueous extract of this leaf has a suitable property for a larvicidal natural product, and may replace harmful chemical pesticides.http://wjst.wu.ac.th/index.php/wjst/article/view/1455LeafAndrographis paniculataCulex quinquefasciatusMosquitoLarvaHistology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wannee JIRAUNGKOORSKUL |
spellingShingle |
Wannee JIRAUNGKOORSKUL Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva Walailak Journal of Science and Technology Leaf Andrographis paniculata Culex quinquefasciatus Mosquito Larva Histology |
author_facet |
Wannee JIRAUNGKOORSKUL |
author_sort |
Wannee JIRAUNGKOORSKUL |
title |
Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva |
title_short |
Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva |
title_full |
Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva |
title_fullStr |
Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva |
title_full_unstemmed |
Larvicidal and Histopathological Effects of Andrographis paniculata Leaf Extract against Culex quinquefasciatus Larva |
title_sort |
larvicidal and histopathological effects of andrographis paniculata leaf extract against culex quinquefasciatus larva |
publisher |
Walailak University |
series |
Walailak Journal of Science and Technology |
issn |
1686-3933 2228-835X |
publishDate |
2016-02-01 |
description |
Andrographis paniculata leaf aqueous extract over 0.5, 1, 3, 5, and 24 h were determined to contain the highest amount of total phenolic compound and was used to evaluate mosquito larvicidal properties against Culex quinquefasciatus at varies concentrations (3.125, 6.25, 12.5, and 25 ppm) by determining the median and 90 % lethal concentration, LC50 and LC90, respectively, within 24 h and by histological analysis. The results revealed that the total phenolic compound measurement in each time extraction were 7.97, 8.68, 7.16, 6.70, and 5.78 mg/g GAE, respectively. The one hour aqueous extract of A. paniculata leaf expressed the 24-h LC50 and LC90 values in C. quinquefasciatus which were 15.93 and 28.23 ppm, respectively. Dose dependent lesions were observed. Under histological analysis, midgut lesions, i.e., separation of the epithelial cells from the basement membrane, elongation protruding into the lumen, disruption of the brush border, and the appearance of several vesicles and cytoplasm masses were observed in this study. The present study reveals that the aqueous extract of this leaf has a suitable property for a larvicidal natural product, and may replace harmful chemical pesticides. |
topic |
Leaf Andrographis paniculata Culex quinquefasciatus Mosquito Larva Histology |
url |
http://wjst.wu.ac.th/index.php/wjst/article/view/1455 |
work_keys_str_mv |
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