Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis
Abstract Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples followi...
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2017-08-01
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doaj-63c417934829441eb4e5ebde576ab3af2020-12-08T00:46:05ZengNature Publishing GroupScientific Reports2045-23222017-08-01711810.1038/s41598-017-07564-3Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosisYongchun Shen0Xiaorong Zhang1Wenyi Lin2Chun Wan3Qiyuan Li4Yong Jiang5Department of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of ChinaDepartment of Pathology, The First Affiliated Hospital of Jiujiang UniversityDepartment of Pathology, Chengdu Womens’ and Children’s Central HospitalDepartment of Respiratory and Critical Care Medicine, West China Hospital of Sichuan University and Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of ChinaDepartment of Pathology, West China Hospital of Sichuan UniversityDepartment of Pathology, West China Hospital of Sichuan UniversityAbstract Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples following Papanicolaou’s or Special staining with Grocott’s methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n = 54) and sputum samples (n = 117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT. LCT slides were generally superior to conventional slides, showing smaller cell monolayer surface area, clearer background and more distinct stereoscopic cytological features. For Papanicolaou’s staining, LCT-prepared slides allowed a higher positive rate of Aspergillus identification than conventional slides for bronchial brushing samples (59.25% vs. 20.37%, P < 0.05) and sputum samples (29.05% vs. 8.55%, P < 0.05). Similarly, Special staining of LCT-prepared slides showed a higher positive rate of Aspergillus identification for bronchial brushing samples (83.33% vs. 57.41%, P < 0.05) and sputum samples (43.59% vs. 19.66%, P < 0.05). This preliminary study suggests that LCT may be better than conventional slide preparation for identifying Aspergillus in respiratory samples from patients with pulmonary aspergillosis.https://doi.org/10.1038/s41598-017-07564-3 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yongchun Shen Xiaorong Zhang Wenyi Lin Chun Wan Qiyuan Li Yong Jiang |
spellingShingle |
Yongchun Shen Xiaorong Zhang Wenyi Lin Chun Wan Qiyuan Li Yong Jiang Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis Scientific Reports |
author_facet |
Yongchun Shen Xiaorong Zhang Wenyi Lin Chun Wan Qiyuan Li Yong Jiang |
author_sort |
Yongchun Shen |
title |
Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis |
title_short |
Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis |
title_full |
Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis |
title_fullStr |
Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis |
title_full_unstemmed |
Liquid-based cytopathology test as a novel method to identify Aspergillus in patients with pulmonary aspergillosis |
title_sort |
liquid-based cytopathology test as a novel method to identify aspergillus in patients with pulmonary aspergillosis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-08-01 |
description |
Abstract Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples following Papanicolaou’s or Special staining with Grocott’s methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n = 54) and sputum samples (n = 117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT. LCT slides were generally superior to conventional slides, showing smaller cell monolayer surface area, clearer background and more distinct stereoscopic cytological features. For Papanicolaou’s staining, LCT-prepared slides allowed a higher positive rate of Aspergillus identification than conventional slides for bronchial brushing samples (59.25% vs. 20.37%, P < 0.05) and sputum samples (29.05% vs. 8.55%, P < 0.05). Similarly, Special staining of LCT-prepared slides showed a higher positive rate of Aspergillus identification for bronchial brushing samples (83.33% vs. 57.41%, P < 0.05) and sputum samples (43.59% vs. 19.66%, P < 0.05). This preliminary study suggests that LCT may be better than conventional slide preparation for identifying Aspergillus in respiratory samples from patients with pulmonary aspergillosis. |
url |
https://doi.org/10.1038/s41598-017-07564-3 |
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