Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice
Perfluorooctanoic acid (PFOA) is a widely used perfluorinated compound and known to cause developmental toxicity which includes the increase of resorbed embryo, decrease of fetal survival, and fetal growth retardation. Nevertheless, whether it is associated with alteration of placental development r...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-02-01
|
Series: | Frontiers in Physiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00051/full |
id |
doaj-63e0740b294144b08d23c19044d43e78 |
---|---|
record_format |
Article |
spelling |
doaj-63e0740b294144b08d23c19044d43e782020-11-25T03:48:44ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-02-011110.3389/fphys.2020.00051491307Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in MiceWenyu Jiang0Wenyu Jiang1Yu Deng2Yu Deng3Zifan Song4Yajuan Xie5Lixin Gong6Yilu Chen7Haibin Kuang8Haibin Kuang9Department of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaDepartment of Clinic Medicine, School of Queen Mary, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaDepartment of Clinic Medicine, School of Queen Mary, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaDepartment of Physiology, Basic Medical College, Nanchang University, Nanchang, ChinaJiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology, Medical Experimental Teaching Center, Nanchang University, Nanchang, ChinaPerfluorooctanoic acid (PFOA) is a widely used perfluorinated compound and known to cause developmental toxicity which includes the increase of resorbed embryo, decrease of fetal survival, and fetal growth retardation. Nevertheless, whether it is associated with alteration of placental development remains unknown. Pregnant mice were gavaged with 0, 2.5, 5, 10 mg PFOA /kg/day from pregnancy day (PD) 1 to PD 13. Results showed that PFOA exposure markedly decreased the placental weight and caused interstitial edema of placenta. Laminin staining indicated that blood sinusoids area was shrunken in placenta of PFOA-exposed mice. Furthermore, PFOA treatment significantly reduced numbers of uNK cells. Western blot analysis revealed that levels of Bax and cleaved-caspase 3 proteins were markedly up-regulated in PFOA-treated groups. In addition, TEM examination showed that PFOA treatment caused rupture of nuclear membrane and nuclear pyknosis and fragmentation. Thus, our results suggested that gestational PFOA exposure significantly inhibited development of early placenta through shrinkage of labyrinth vessels and downregulation of uNK cells and apoptosis induction, which may result in adverse gestational outcomes.https://www.frontiersin.org/article/10.3389/fphys.2020.00051/fullperfluorooctanoic acidplacental developmenttoxicityapoptosisuNK cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wenyu Jiang Wenyu Jiang Yu Deng Yu Deng Zifan Song Yajuan Xie Lixin Gong Yilu Chen Haibin Kuang Haibin Kuang |
spellingShingle |
Wenyu Jiang Wenyu Jiang Yu Deng Yu Deng Zifan Song Yajuan Xie Lixin Gong Yilu Chen Haibin Kuang Haibin Kuang Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice Frontiers in Physiology perfluorooctanoic acid placental development toxicity apoptosis uNK cells |
author_facet |
Wenyu Jiang Wenyu Jiang Yu Deng Yu Deng Zifan Song Yajuan Xie Lixin Gong Yilu Chen Haibin Kuang Haibin Kuang |
author_sort |
Wenyu Jiang |
title |
Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice |
title_short |
Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice |
title_full |
Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice |
title_fullStr |
Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice |
title_full_unstemmed |
Gestational Perfluorooctanoic Acid Exposure Inhibits Placental Development by Dysregulation of Labyrinth Vessels and uNK Cells and Apoptosis in Mice |
title_sort |
gestational perfluorooctanoic acid exposure inhibits placental development by dysregulation of labyrinth vessels and unk cells and apoptosis in mice |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2020-02-01 |
description |
Perfluorooctanoic acid (PFOA) is a widely used perfluorinated compound and known to cause developmental toxicity which includes the increase of resorbed embryo, decrease of fetal survival, and fetal growth retardation. Nevertheless, whether it is associated with alteration of placental development remains unknown. Pregnant mice were gavaged with 0, 2.5, 5, 10 mg PFOA /kg/day from pregnancy day (PD) 1 to PD 13. Results showed that PFOA exposure markedly decreased the placental weight and caused interstitial edema of placenta. Laminin staining indicated that blood sinusoids area was shrunken in placenta of PFOA-exposed mice. Furthermore, PFOA treatment significantly reduced numbers of uNK cells. Western blot analysis revealed that levels of Bax and cleaved-caspase 3 proteins were markedly up-regulated in PFOA-treated groups. In addition, TEM examination showed that PFOA treatment caused rupture of nuclear membrane and nuclear pyknosis and fragmentation. Thus, our results suggested that gestational PFOA exposure significantly inhibited development of early placenta through shrinkage of labyrinth vessels and downregulation of uNK cells and apoptosis induction, which may result in adverse gestational outcomes. |
topic |
perfluorooctanoic acid placental development toxicity apoptosis uNK cells |
url |
https://www.frontiersin.org/article/10.3389/fphys.2020.00051/full |
work_keys_str_mv |
AT wenyujiang gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT wenyujiang gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT yudeng gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT yudeng gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT zifansong gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT yajuanxie gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT lixingong gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT yiluchen gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT haibinkuang gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice AT haibinkuang gestationalperfluorooctanoicacidexposureinhibitsplacentaldevelopmentbydysregulationoflabyrinthvesselsandunkcellsandapoptosisinmice |
_version_ |
1724497453650018304 |