The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier
Escherichia coli can cause intestinal diseases in humans and livestock, destroy the intestinal barrier, exacerbate systemic inflammation, and seriously threaten human health and animal husbandry development. The aim of this study was to investigate whether the antimicrobial peptide mastoparan X (MPX...
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Frontiers Media S.A.
2021-06-01
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Series: | Frontiers in Microbiology |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.644887/full |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xueqin Zhao Xueqin Zhao Lei Wang Lei Wang Chunling Zhu Xiaojing Xia Shouping Zhang Yimin Wang Huihui Zhang Yanzhao Xu Shijun Chen Jinqing Jiang Shanqin Liu Yundi Wu Xilong Wu Gaiping Zhang Yueyu Bai Hanna Fotina Jianhe Hu |
spellingShingle |
Xueqin Zhao Xueqin Zhao Lei Wang Lei Wang Chunling Zhu Xiaojing Xia Shouping Zhang Yimin Wang Huihui Zhang Yanzhao Xu Shijun Chen Jinqing Jiang Shanqin Liu Yundi Wu Xilong Wu Gaiping Zhang Yueyu Bai Hanna Fotina Jianhe Hu The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier Frontiers in Microbiology antimicrobial peptide mastoparan X inflammation intestinal barrier Enterohemorrhagic Escherichia coli O157:H7 mice |
author_facet |
Xueqin Zhao Xueqin Zhao Lei Wang Lei Wang Chunling Zhu Xiaojing Xia Shouping Zhang Yimin Wang Huihui Zhang Yanzhao Xu Shijun Chen Jinqing Jiang Shanqin Liu Yundi Wu Xilong Wu Gaiping Zhang Yueyu Bai Hanna Fotina Jianhe Hu |
author_sort |
Xueqin Zhao |
title |
The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier |
title_short |
The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier |
title_full |
The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier |
title_fullStr |
The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier |
title_full_unstemmed |
The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial Barrier |
title_sort |
antimicrobial peptide mastoparan x protects against enterohemorrhagic escherichia coli o157:h7 infection, inhibits inflammation, and enhances the intestinal epithelial barrier |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-06-01 |
description |
Escherichia coli can cause intestinal diseases in humans and livestock, destroy the intestinal barrier, exacerbate systemic inflammation, and seriously threaten human health and animal husbandry development. The aim of this study was to investigate whether the antimicrobial peptide mastoparan X (MPX) was effective against E. coli infection. BALB/c mice infected with E. coli by intraperitoneal injection, which represents a sepsis model. In this study, MPX exhibited no toxicity in IPEC-J2 cells and notably suppressed the levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), myeloperoxidase (MPO), and lactate dehydrogenase (LDH) released by E. coli. In addition, MPX improved the expression of ZO-1, occludin, and claudin and enhanced the wound healing of IPEC-J2 cells. The therapeutic effect of MPX was evaluated in a murine model, revealing that it protected mice from lethal E. coli infection. Furthermore, MPX increased the length of villi and reduced the infiltration of inflammatory cells into the jejunum. SEM and TEM analyses showed that MPX effectively ameliorated the jejunum damage caused by E. coli and increased the number and length of microvilli. In addition, MPX decreased the expression of IL-2, IL-6, TNF-α, p-p38, and p-p65 in the jejunum and colon. Moreover, MPX increased the expression of ZO-1, occludin, and MUC2 in the jejunum and colon, improved the function of the intestinal barrier, and promoted the absorption of nutrients. This study suggests that MPX is an effective therapeutic agent for E. coli infection and other intestinal diseases, laying the foundation for the development of new drugs for bacterial infections. |
topic |
antimicrobial peptide mastoparan X inflammation intestinal barrier Enterohemorrhagic Escherichia coli O157:H7 mice |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.644887/full |
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doaj-63fd5dd5c07f48878f7d6a0618bfafe22021-06-10T06:45:44ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.644887644887The Antimicrobial Peptide Mastoparan X Protects Against Enterohemorrhagic Escherichia coli O157:H7 Infection, Inhibits Inflammation, and Enhances the Intestinal Epithelial BarrierXueqin Zhao0Xueqin Zhao1Lei Wang2Lei Wang3Chunling Zhu4Xiaojing Xia5Shouping Zhang6Yimin Wang7Huihui Zhang8Yanzhao Xu9Shijun Chen10Jinqing Jiang11Shanqin Liu12Yundi Wu13Xilong Wu14Gaiping Zhang15Yueyu Bai16Hanna Fotina17Jianhe Hu18College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaFaculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, UkraineCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, School of Biomedical Engineering, Hainan University, Haikou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaSchool of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, School of Biomedical Engineering, Hainan University, Haikou, ChinaState Key Laboratory of Marine Resource Utilization in South China Sea, School of Biomedical Engineering, Hainan University, Haikou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaFaculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, UkraineCollege of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, ChinaEscherichia coli can cause intestinal diseases in humans and livestock, destroy the intestinal barrier, exacerbate systemic inflammation, and seriously threaten human health and animal husbandry development. The aim of this study was to investigate whether the antimicrobial peptide mastoparan X (MPX) was effective against E. coli infection. BALB/c mice infected with E. coli by intraperitoneal injection, which represents a sepsis model. In this study, MPX exhibited no toxicity in IPEC-J2 cells and notably suppressed the levels of interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), myeloperoxidase (MPO), and lactate dehydrogenase (LDH) released by E. coli. In addition, MPX improved the expression of ZO-1, occludin, and claudin and enhanced the wound healing of IPEC-J2 cells. The therapeutic effect of MPX was evaluated in a murine model, revealing that it protected mice from lethal E. coli infection. Furthermore, MPX increased the length of villi and reduced the infiltration of inflammatory cells into the jejunum. SEM and TEM analyses showed that MPX effectively ameliorated the jejunum damage caused by E. coli and increased the number and length of microvilli. In addition, MPX decreased the expression of IL-2, IL-6, TNF-α, p-p38, and p-p65 in the jejunum and colon. Moreover, MPX increased the expression of ZO-1, occludin, and MUC2 in the jejunum and colon, improved the function of the intestinal barrier, and promoted the absorption of nutrients. This study suggests that MPX is an effective therapeutic agent for E. coli infection and other intestinal diseases, laying the foundation for the development of new drugs for bacterial infections.https://www.frontiersin.org/articles/10.3389/fmicb.2021.644887/fullantimicrobial peptide mastoparan Xinflammationintestinal barrierEnterohemorrhagic Escherichia coli O157:H7mice |