| Summary: | ABSTRACT: Objectives: Vibrio parahaemolyticus ST165 strain (FB-11) was first isolated in 2019 in China from Penaeus vannamei from seawater farms. The strain includes multidrug resistant (MDR) and multi-heavy metal resistant (MHMR) phenotypes. In this study, we aimed to determine the draft genome sequence of strain FB-11 and analyze the genetic features with a special focus on MDR and MHMR genes. Methods: The genomic DNA was sequenced using Nanopore PromethION and the Illumina Novaseq6000 platform, and the reads were de novo assembled into contigs using Unicycler. Genome function elements were predicted, the coding sequences were annotated, and whole-genome sequencing (WGS) analysis was performed. Results: WGS analysis revealed that the genome comprised two chromosomes of 3,328,286 bp (GC content, 45.37%) and 1,805,825 bp (GC content, 45.36%). It harbored 26 important drug resistant genes and six important heavy metal resistant genes; all of these were located on the chromosomes. Multilocus sequence type of the strain was ST165. Conclusions: This is the first report of the complete genome sequence of a V. parahaemolyticus ST165 strain isolated from P. vannamei in China. This genome sequence provides useful information on the genomic features associated with antimicrobial and heavy metal resistance in V. parahaemolyticus ST165.
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