Fractionation of phenolase from green table olives (Ascolana tenera var.) by immobilized copper affinity chromatography
Phenolase from green table olives (Ascolana tenera var.) was fractionated by immobilized copper affinity chromatography. Four chromatographic fractions with high specific activities were obtained. The highest activity was found for the fraction eluted with L-histidine buffer. The four active fractio...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Consejo Superior de Investigaciones Científicas
1995-10-01
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Series: | Grasas y Aceites |
Subjects: | |
Online Access: | http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/933 |
Summary: | Phenolase from green table olives (Ascolana tenera var.) was fractionated by immobilized copper affinity chromatography. Four chromatographic fractions with high specific activities were obtained. The highest activity was found for the fraction eluted with L-histidine buffer. The four active fractions were characterized by. their response to both specificity and concentration of the substrate. |
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ISSN: | 0017-3495 1988-4214 |