Fractionation of phenolase from green table olives (Ascolana tenera var.) by immobilized copper affinity chromatography

• Main Authors: V. Sciancalepore, G. De Stefano
• Format: Article
• Language: English
• Published: Consejo Superior de Investigaciones Científicas 1995-10-01
• Series: Grasas y Aceites
• Subjects: ascolana tenera var; green table olive; immobilized copper affinity chromatography; phenolase (fractionation)
• Online Access: http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/933

Phenolase from green table olives (Ascolana tenera var.) was fractionated by immobilized copper affinity chromatography. Four chromatographic fractions with high specific activities were obtained. The highest activity was found for the fraction eluted with L-histidine buffer. The four active fractions were characterized by. their response to both specificity and concentration of the substrate.