Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro
The aim of the study was to examine the efficacy of cold atmospheric plasma (CAP) on the mineralization and cell proliferation of murine dental cementoblasts. Cells were treated with CAP and enamel matrix derivates (EMD). Gene expression of alkaline phosphatase (ALP), bone gamma-carboxyglutamate pro...
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doaj-64300049f57b4325b1c2ba25496470262021-06-01T00:17:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01225280528010.3390/ijms22105280Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In VitroBenedikt Eggers0Jana Marciniak1James Deschner2Matthias Bernhard Stope3Alexander Mustea4Franz-Josef Kramer5Marjan Nokhbehsaim6Department of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Orthodontics, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Periodontology and Operative Dentistry, University Medical Center of the Johannes Gutenberg University, 55131 Mainz, GermanyDepartment of Gynecology and Gynecological Oncology, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Gynecology and Gynecological Oncology, University Hospital Bonn, 53111 Bonn, GermanyDepartment of Oral, Maxillofacial and Plastic Surgery, University Hospital Bonn, 53111 Bonn, GermanySection of Experimental Dento-Maxillo-Facial Medicine, University Hospital Bonn, 53111 Bonn, GermanyThe aim of the study was to examine the efficacy of cold atmospheric plasma (CAP) on the mineralization and cell proliferation of murine dental cementoblasts. Cells were treated with CAP and enamel matrix derivates (EMD). Gene expression of alkaline phosphatase (ALP), bone gamma-carboxyglutamate protein (BGLAP), periostin (POSTN), osteopontin (OPN), osterix (OSX), collagen type I alpha 1 chain (COL1A1), dentin matrix acidic phosphoprotein (DMP)1, RUNX family transcription factor (RUNX)2, and marker of proliferation Ki-67 (KI67) was quantified by real-time PCR. Protein expression was analyzed by immunocytochemistry and ELISA. ALP activity was determined by ALP assay. Von Kossa and alizarin red staining were used to display mineralization. Cell viability was analyzed by XTT assay, and morphological characterization was performed by DAPI/phalloidin staining. Cell migration was quantified with an established scratch assay. CAP and EMD upregulated both mRNA and protein synthesis of ALP, POSTN, and OPN. Additionally, DMP1 and COL1A1 were upregulated at both gene and protein levels. In addition to upregulated RUNX2 mRNA levels, treated cells mineralized more intensively. Moreover, CAP treatment resulted in an upregulation of KI67, higher cell viability, and improved cell migration. Our study shows that CAP appears to have stimulatory effects on regeneration-associated cell functions in cementoblasts.https://www.mdpi.com/1422-0067/22/10/5280cold atmospheric plasmaemdogaincementoblastsmineralizationproliferationtraumatic dental injuries |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Benedikt Eggers Jana Marciniak James Deschner Matthias Bernhard Stope Alexander Mustea Franz-Josef Kramer Marjan Nokhbehsaim |
spellingShingle |
Benedikt Eggers Jana Marciniak James Deschner Matthias Bernhard Stope Alexander Mustea Franz-Josef Kramer Marjan Nokhbehsaim Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro International Journal of Molecular Sciences cold atmospheric plasma emdogain cementoblasts mineralization proliferation traumatic dental injuries |
author_facet |
Benedikt Eggers Jana Marciniak James Deschner Matthias Bernhard Stope Alexander Mustea Franz-Josef Kramer Marjan Nokhbehsaim |
author_sort |
Benedikt Eggers |
title |
Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro |
title_short |
Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro |
title_full |
Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro |
title_fullStr |
Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro |
title_full_unstemmed |
Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro |
title_sort |
cold atmospheric plasma promotes regeneration-associated cell functions of murine cementoblasts in vitro |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-05-01 |
description |
The aim of the study was to examine the efficacy of cold atmospheric plasma (CAP) on the mineralization and cell proliferation of murine dental cementoblasts. Cells were treated with CAP and enamel matrix derivates (EMD). Gene expression of alkaline phosphatase (ALP), bone gamma-carboxyglutamate protein (BGLAP), periostin (POSTN), osteopontin (OPN), osterix (OSX), collagen type I alpha 1 chain (COL1A1), dentin matrix acidic phosphoprotein (DMP)1, RUNX family transcription factor (RUNX)2, and marker of proliferation Ki-67 (KI67) was quantified by real-time PCR. Protein expression was analyzed by immunocytochemistry and ELISA. ALP activity was determined by ALP assay. Von Kossa and alizarin red staining were used to display mineralization. Cell viability was analyzed by XTT assay, and morphological characterization was performed by DAPI/phalloidin staining. Cell migration was quantified with an established scratch assay. CAP and EMD upregulated both mRNA and protein synthesis of ALP, POSTN, and OPN. Additionally, DMP1 and COL1A1 were upregulated at both gene and protein levels. In addition to upregulated RUNX2 mRNA levels, treated cells mineralized more intensively. Moreover, CAP treatment resulted in an upregulation of KI67, higher cell viability, and improved cell migration. Our study shows that CAP appears to have stimulatory effects on regeneration-associated cell functions in cementoblasts. |
topic |
cold atmospheric plasma emdogain cementoblasts mineralization proliferation traumatic dental injuries |
url |
https://www.mdpi.com/1422-0067/22/10/5280 |
work_keys_str_mv |
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